PAK2 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 85646-2-RR (same clone as 85646-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85646-2-RR (PAK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85646-2-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 85646-2-RR (same clone as 85646-2-PBS)

  IP result of anti-PAK2 (IP:85646-2-RR, 4ug; Detection:85646-2-RR 1:1000) with HeLa cells lysate 920 ug. This data was developed using the same antibody clone with 85646-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 85646-2-RR (same clone as 85646-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PAK2 antibody (85646-2-RR, Clone: 243124G9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85646-2-PBS in a different storage buffer formulation.

PAK2 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA

Cat No. 85646-2-PBS

Clone No.243124G9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

C t PAK2, EC:2.7.11.1, Gamma PAK, Gamma-PAK, p21 activated kinase 2

---

Product Information

85646-2-PBS targets PAK2 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: p21 protein (Cdc42/Rac)-activated kinase 2
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: NM_002577
• Gene Symbol: PAK2
• Gene ID (NCBI): 5062
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13177
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PAK2, also named as PAK65, PAKgamma, p58, PAK-2p27, PAK-2p24 and C-t-PAK2, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. PAK2 has several isoforms with the MW of 54-62 kDa and 41 kDa. The 62 kDa PAK2 is cleaved into a 34 kDa C terminal fragment and a 28 kDa N terminal fragment with a time course that parallels apoptotic death in Jurkat cells. (PMID:10200518). Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form.