Tested Applications
| Positive WB detected in | COLO 320 cells, HeLa cells, PC-3 cells, rat skin tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14685-1-AP targets PAK4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5981 Product name: Recombinant human PAK4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC025282 Sequence: MFGKRKKRVEISAPSNFEHRVHTGFDQHEQKFTGLPRQWQSLIEESARRPKPLVDPACITSIQPGAPKTIVRGSKGAKDGALTLLLDEFENMSVTRSNSLRRDSPPPPARARQENGMPEEPATTARGGPGKAGSRGRFAGHSEAGGGSGDRRRAGPEKRPKSSREGSGGPQESSRDKRPLSGPDVGTPQPAGLASGAKLAAGRPFNTYPRADTDHPSRGAQGEPHDVAPNGPSAGGLAIPQSSSSSSRPPTRARGAPSPGVLGPHASEPQLAPPACTPAAPAVPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLDNFIKIGEGSTGIVCIATVRSSGKLVAVK Predict reactive species |
| Full Name | p21 protein (Cdc42/Rac)-activated kinase 4 |
| Calculated Molecular Weight | 64 kDa |
| Observed Molecular Weight | 64 kDa |
| GenBank Accession Number | BC025282 |
| Gene Symbol | PAK4 |
| Gene ID (NCBI) | 10298 |
| RRID | AB_2158610 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O96013 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PAK4(p21-activated kinase 4) is also named as KIAA1142. It belongs to the protein kinase superfamily.PAK4 regulates cell morphology, cytoskeletal organization, and cell proliferation and migration. It can also function as an antiapoptotic protein (PMID:14517283). It has 4 isoforms produced by alternative splicing. And it can be autophosphorylated on serine residues when activated by CDC42/p21 (PMID:11278822).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PAK4 antibody 14685-1-AP | Download protocol |
| IHC protocol for PAK4 antibody 14685-1-AP | Download protocol |
| IP protocol for PAK4 antibody 14685-1-AP | Download protocol |
| WB protocol for PAK4 antibody 14685-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PAK4 antibody (Cat# 14685-1-AP) has accumulated 29 citations published in high-impact journals including Nature Cancer, Nature Communications, Cancer Research, Cell Reports, Communications Biology, Acta Pharmaceutica Sinica B, and Journal of Biological Chemistry. Associated research fields encompass oncology (pancreatic, breast, ovarian, colorectal, prostate, and other cancers), immunotherapy, inflammatory responses, neurodevelopment, cell polarity, and reproductive biology.
| Species | Application | Title |
|---|---|---|
Cancer Res Targeting PAK4 Inhibits Ras-Mediated Signaling and Multiple Oncogenic Pathways in High-Risk Rhabdomyosarcoma. | ||
Bone Res Pak4-mediated crosstalk between necroptotic macrophages and tendon stem/progenitor cells contributes to traumatic heterotopic ossification formation. | ||
Nat Commun Proximity proteomics identifies PAK4 as a component of Afadin-Nectin junctions.
| ||
Acta Pharm Sin B Design, synthesis and pharmacological evaluation of 1,2,3,4-tetrahydrobenzofuro[2,3-c]pyridine derivatives as p21-activated kinase 4 inhibitors for treatment of pancreatic cancer | ||
Adv Sci (Weinh) Smoking-Induced STC2(+) Tumor Cells Drive Tumor-Vascular Crosstalk in Laryngeal Squamous Cell Carcinoma via Spatial and Single-Cell Transcriptomics. |
Reviews
What customers say
Both reviewers gave 5-star ratings. One user tested the antibody across multiple applications—Western Blot, IHC, IF, IP, Flow Cytometry, and cell culture—on human cells and reported it works well. The other user validated it at 1:1000 dilution on breast cancer cells for Western Blot and Immunofluorescence, noting a strong signal by WB. Overall, users are satisfied with the antibody's performance and signal strength across various applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Juan (Verified Customer) (05-21-2024) | This primary antibody works pretty good!
|
Emily (Verified Customer) (07-18-2022) | Good strong signal by western blot, intend to use for IF soon
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