PAK6 Polyclonal antibody, PBS Only

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• PAK6 Polyclonal antibody, PBS Only (13539-1-PBS)

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  WB analysis using 13539-1-AP (same clone as 13539-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13539-1-AP (PAK6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13539-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 13539-1-AP (same clone as 13539-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 13539-1-AP (PAK6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13539-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 13539-1-AP (same clone as 13539-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13539-1-AP (PAK6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13539-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 13539-1-AP (same clone as 13539-1-PBS)

  IP result of anti-PAK6 (IP:13539-1-AP, 4ug; Detection:13539-1-AP 1:300) with mouse testis tissue lysate 4000 ug. This data was developed using the same antibody clone with 13539-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 13539-1-AP (same clone as 13539-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13539-1-AP (PAK6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13539-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 13539-1-AP (same clone as 13539-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13539-1-AP (PAK6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13539-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 13539-1-AP (same clone as 13539-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PAK6 antibody (13539-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13539-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PAK6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 13539-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

PAK5, PAK 6, PAK 5, p21-activated kinase 6, p21 activated kinase 6

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Product Information

13539-1-PBS targets PAK6 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4467

  Product name: Recombinant human PAK6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 330-681 aa of BC035596

  Sequence: QKSLRTAPATGQLPGRSSPAGSPRTWHAQISTSNLYLPQDPTVAKGALAGEDTGVVTHEQFKAALRMVVDQGDPRLLLDSYVKIGEGSTGIVCLAREKHSGRQVAVKMMDLRKQQRRELLFNEVVIMRDYQHFNVVEMYKSYLVGEELWVLMEFLQGGALTDIVSQVRLNEEQIATVCEAVLQALAYLHAQGVIHRDIKSDSILLTLDGRVKLSDFGFCAQISKDVPKRKSLVGTPYWMAPEVISRSLYATEVDIWSLGIMVIEMVDGEPPYFSDSPVQAMKRLRDSPPPKLKNSHKVSPVLRDFLERMLVRDPQERATAQELLDHPFLLQTGLPECLVPLIQLYRKQTSTC

• Full Name: p21 protein (Cdc42/Rac)-activated kinase 6
• Calculated Molecular Weight: 681 aa, 75 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC035596
• Gene Symbol: PAK6
• Gene ID (NCBI): 56924
• RRID: AB_2158467
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NQU5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PAK6(p21-activated kinase 6) is a serine/threonine kinase belonging to the p21-activated kinase (PAK) family. .This protein plays a role in the regulation of gene transcription and may protect cells from apoptosis through phosphorylation of BAD. Northern blot analysis revealed that PAK6 is highly expressed in testis and prostate tissues (PMID:11278661). The immunogen of this antibody is the C-terminal of PAK6. This antibody has weak cross-reaction with other PAK members.