PALMD Polyclonal antibody

• Featured Product
• KD/KO Validated

PALMD Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 16531-1-AP

Synonyms

C1orf11, PALMD, palmdelphin, PALML, Paralemnin like protein

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Validation Data Gallery

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  WB analysis of HeLa using 16531-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16531-1-AP (PALMD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 16531-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16531-1-AP (PALMD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16531-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16531-1-AP (PALMD antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment using 16531-1-AP

  IP result of PALMD antibody (16531-1-AP for Ip and Detection) with mouse heart tissue lysate.

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  IP experiment of mouse heart using 16531-1-AP

  IP result of anti-PALMD (IP:16531-1-AP, 5ug; Detection:16531-1-AP 1:500) with mouse heart tissue lysate 6000ug.

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  IHC staining of human liver using 16531-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16531-1-AP (PALMD antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 16531-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16531-1-AP (PALMD antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse lung tissue
• Positive IP detected in: mouse heart tissue,
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

16531-1-AP targets PALMD in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PALMD fusion protein Ag9796
• Full Name: palmdelphin
• Calculated Molecular Weight: 551 aa, 63 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC012570
• Gene Symbol: PALMD
• Gene ID (NCBI): 54873
• RRID: AB_2236613
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Palmdelphin, encoded by PALMD gene, is a newly identified cytosolic isoform of paralemmin-1, a lipid raft-associated protein implicated in cell shape control. Paralemmin (Palm) is a prenyl-palmitoyl anchored membrane protein that can drive membrane and process formation in neurons. Previous finding suggest that palmdelphin may peripherally associate with endomembranes or cytoskeleton-linked structures. Palmdelphin appears to be 80 kDa in Western blotting, larger than the predicated of 63 kDa, and this may be due to the acidic nature of this protein or its posttranslational modification.

Publications

Species	Application	Title
mouse	WB	Hum Mol Genet; Interactome network analysis identifies multiple caspase-6 interactors involved in the pathogenesis of HD.; Authors - Sean-Patrick Riechers
human	WB	Cell Death Dis; Palmdelphin, a novel target of p53 with Ser46 phosphorylation, controls cell death in response to DNA damage.; Authors - N Dashzeveg ; KD Validated
rat	WB,IF	Front Cell Neurosci; Role of a Pdlim5:PalmD complex in directing dendrite morphology; Authors - Yogesh Srivastava
human	WB	Front Oncol; Low Level of PALMD Contributes to the Metastasis of Uveal Melanoma.; Authors - Min-Yun Cai ; KD Validated
human	WB,IF	bioRxiv; Role of a Pdlim5:PalmD complex in directing dendrite morphology; Authors - Yogesh Srivastava
mouse	IF,IHC	Cell Rep; Integrative multi-omic profiling of adult mouse brain endothelial cells and potential implications in Alzheimer's disease; Authors - Min Yu