PAM Polyclonal antibody

PAM Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 26972-1-AP

Synonyms

PAL, PAM, Peptidylamidoglycolate lyase, PHM

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Validation Data Gallery

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  WB analysis of U2OS using 26972-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 26972-1-AP (PAM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 26972-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26972-1-AP (PAM Antibody) at dilution of 1:200 (under 10x lens).

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Tested Applications

• Positive WB detected in: U2OS cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 26972-1-AP is PAM Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PAM fusion protein Ag25719
• Full Name: peptidylglycine alpha-amidating monooxygenase
• Calculated Molecular Weight: 108 kDa
• Observed Molecular Weight: 110-120 kDa
• GenBank Accession Number: BC018127
• Gene Symbol: PAM
• Gene ID (NCBI): 5066
• RRID: AB_2880706
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
hamster	WB	Comp Biochem Physiol B Biochem Mol Biol; Change on apoptosis, autophagy and mitochondria of the Harderian gland in Cricetulus barabensis during age.; Authors - Xiao-Tong Kong