PAPOLB Polyclonal antibody

PAPOLB Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12821-1-AP

Synonyms

PAP beta, PAPOLB, PAPT, Poly(A) polymerase beta, TPAP

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Validation Data Gallery

• 

  WB analysis of mouse testis using 12821-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12821-1-AP (PAPOLB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12821-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12821-1-AP (PAPOLB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12821-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12821-1-AP (PAPOLB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12821-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12821-1-AP (PAPOLB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 12821-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 12821-1-AP (PAPOLB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 12821-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12821-1-AP (PAPOLB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12821-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12821-1-AP (PAPOLB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse testis tissue, human kidney tissue, HeLa cells, human brain tissue, human stomach tissue, mouse ovary tissue, human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12821-1-AP targets PAPOLB in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PAPOLB fusion protein Ag3487
• Full Name: poly(A) polymerase beta (testis specific)
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 71 kDa
• GenBank Accession Number: BC036653
• Gene Symbol: PAPOLB
• Gene ID (NCBI): 56903
• RRID: AB_2159217
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.