Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:800-1:3200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24284-1-AP targets PAPOLG in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16208 Product name: Recombinant human PAPOLG protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 469-736 aa of BC111701 Sequence: QANNINMLKEGMKIEATHVKKKQLHHYLPAEILQKKKKQSLSDVNRSSGGLQSKRLSLDSSCLDSSRDTDNGTPFNSPASKSDSPSVGETERNSAEPAAVIVEKPLSVPPAQGLSIPVIGAKVDSTVKTVSPPTVCTIPTVVGRNVIPRITTPHNPAQGQPHLNGMSNITKTVTPKRSHSPSIDGTPKRLKDVEKFIRLESTFKDPRTAEERKRKSVDAIGGESMPIPTIDTSRKKRLPSKELPDSSSPVPANNIRVIKNSIRLTLNR Predict reactive species |
| Full Name | poly(A) polymerase gamma |
| Calculated Molecular Weight | 736 aa, 83 kDa |
| Observed Molecular Weight | 83 kDa |
| GenBank Accession Number | BC111701 |
| Gene Symbol | PAPOLG |
| Gene ID (NCBI) | 64895 |
| RRID | AB_2879471 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BWT3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PAPOLG, also named as Neo-poly(A) polymerase or Polynucleotide adenylyltransferase gamma, is a 736 amino acid protein, which belongs to the poly(A) polymerase family. PAPOLG is expressed predominantly in testis, and weakly in other tissues. PAPOLG is responsible for the post-transcriptional adenylation of the 3'-terminal of mRNA precursors and several small RNAs including signal recognition particle (SRP) RNA, nuclear 7SK RNA, U2 small nuclear RNA, and ribosomal 5S RNA.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PAPOLG antibody 24284-1-AP | Download protocol |
| IHC protocol for PAPOLG antibody 24284-1-AP | Download protocol |
| IP protocol for PAPOLG antibody 24284-1-AP | Download protocol |
| WB protocol for PAPOLG antibody 24284-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PAPOLG Polyclonal antibody (Cat# 24284-1-AP) has 1 total citation. It appears in Cell (IF 45.1), published in 2026, in a study titled "Functional atlas of vertebrate viral RNA elements that stabilize RNA and enhance translation." The research field is RNA biology and virology, focusing on how viral RNA elements interact with host poly(A) polymerase machinery to stabilize transcripts and enhance translation across vertebrate species.
| Species | Application | Title |
|---|---|---|
Cell Functional atlas of vertebrate viral RNA elements that stabilize RNA and enhance translation. |
