PARD6B Recombinant monoclonal antibody

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• PARD6B Recombinant monoclonal antibody (86786-1-RR)

• 

  WB analysis using 86786-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86786-1-RR (PARD6B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86786-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86786-1-RR (PARD6B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

PARD6B Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86786-1-RR

Clone No.251629D4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

PAR-6 beta, PAR6B, PAR-6B, Partitioning defective 6 homolog beta

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Tested Applications

• Positive WB detected in: HeLa cells, PC-12 cells, Jurkat cells, HEK-293 cells, T-47D cells, Caki-1 cells, RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86786-1-RR targets PARD6B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag5083

  Product name: Recombinant human PARD6B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-372 aa of BC060847

  Sequence: MNRSHRHGAGSGCLGTMEVKSKFGAEFRRFSLERSKPGKFEEFYGLLQHVHKIPNVDVLVGYADIHGDLLPINNDDNYHKAVSTANPLLRIFIQKKEEADYSAFGTDTLIKKKNVLTNVLRPDNHRKKPHIVISMPQDFRPVSSIIDVDILPETHRRVRLYKYGTEKPLGFYIRDGSSVRVTPHGLEKVPGIFISRLVPGGLAQSTGLLAVNDEVLEVNGIEVSGKSLDQVTDMMIANSRNLIITVRPANQRNNVVRNSRTSGSSGQSTDNSLLGYPQQIEPSFEPEDEDSEEDDIIIEDNGVPQQIPKAVPNTESLESLTQIELSFESGQNGFIPSNEVSLAAIASSSNTEFETHAPDQKLLEEDGTIITL

• Full Name: par-6 partitioning defective 6 homolog beta (C. elegans)
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC060847
• Gene Symbol: PARD6B
• Gene ID (NCBI): 84612
• RRID: AB_3745117
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BYG5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PARD6B (also named PAR6B) is a member of the PAR6 family. PARD6B is known to play a key role in mammary epithelial cell biology. Several studies have been reported which indicate deregulated PARD6B signaling contributes to malignant epithelial cell phenotypes due predominantly to disrupted polymerization and maintenance of tight junctions (PMID: 22957302). The approximately 4.8-kb long PARD6B mRNA was predominantly detected in both adult and fetal kidneys, while much weaker but significant signals were observed in the placenta, lung, and liver (PMID: 11260256). This antibody detects PARD6B with an apparent molecular weight of 50-53 kDa as has been demonstrated by several researches (PMID: 22496418; 25662318).