PARG Monoclonal antibody, PBS Only

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  WB analysis using 67946-1-Ig (same clone as 67946-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67946-1-Ig (PARG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67946-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67946-1-Ig (same clone as 67946-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67946-1-Ig (PARG antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67946-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67946-1-Ig (same clone as 67946-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67946-1-Ig (PARG antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67946-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67946-1-Ig (same clone as 67946-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PARG antibody (67946-1-Ig, Clone: 1H10G10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67946-1-PBS in a different storage buffer formulation.

PARG Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67946-1-PBS

Clone No.1H10G10

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PARG, PARG99

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Product Information

67946-1-PBS targets PARG in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27288

  Product name: Recombinant human PARG protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 183-441 aa of BC052966

  Sequence: SNANIDRSPQNDDHSDTDSEENRDNQQFLTTVKLANAKQTTEDEQAREAKSHQKCSKSCDPGEDCASCQQDEIDVVPESPLSDVGSEDVGTGPKNDNKLTRQESCLGNSPPFEKESEPESPMDVDNSKNSCQDSEADEETSPGFDEQEDGSSSQTANKPSRFQARDADIEFRKRYSTKGGEVRLHFQFEGGESRTGMNDLNAKLPGNISSLNVECRNSKQHGKKDSKITDHFMRLPKAEDRRKEQWETKHQRTERKIPK

• Full Name: poly (ADP-ribose) glycohydrolase
• Calculated Molecular Weight: 111 kDa
• Observed Molecular Weight: 111 kDa
• GenBank Accession Number: BC052966
• Gene Symbol: PARG
• Gene ID (NCBI): 8505
• RRID: AB_2918698
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q86W56
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.