PARL Polyclonal antibody

PARL Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 26679-1-AP

Synonyms

MAMP, PACT, PARL, Pbeta, PRO2207, PSARL, PSARL1, PSENIP2, RHBDS1



Tested Applications

Positive WB detected in3T3-L1 cells, HEK-293 cells, HeLa cells, Neuro-2a cells
Positive IHC detected inhuman prostate cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26679-1-AP is PARL Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PARL fusion protein Ag24789
Full Name presenilin associated, rhomboid-like
Calculated molecular weight 42 kDa
Observed molecular weight 36-42 kDa
GenBank accession numberBC014058
Gene symbol PARL
Gene ID (NCBI) 55486
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PARL (Presenilin associated rhomboid like), is a member of the rhomboid family of intramembrane serine proteases that is localized to the inner mitochondrial membrane. PARL has 2 isoforms produced by alternative splicing with the molecular mass of 42 kDa and 37 kDa. PARL regulates mitochondrial remodeling and apoptosis through regulated substrate proteolysis. Proteolytic processing of PARL may result in the release of a small peptide, P-beta, which may transit to the nucleus. The self-regulated cleavage of PARL at positions 52-53 (α-site) and 77--78 (β-site) produce two cleaved forms of PARL named MAMP and PACT with the molecular mass of 36 kDa and 33 kDa respectively (PMID: 14732705, 28178523).

Protocols

Product Specific Protocols
WB protocol for PARL antibody 26679-1-APDownload protocol
IHC protocol for PARL antibody 26679-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols