PARL Polyclonal antibody

PARL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 26679-1-AP

Synonyms

MAMP, PACT, PARL, Pbeta, PRO2207, PSARL, PSARL1, PSENIP2, RHBDS1

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Validation Data Gallery

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  WB analysis of 3T3-L1 using 26679-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 26679-1-AP (PARL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 26679-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26679-1-AP (PARL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 26679-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 26679-1-AP (PARL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 26679-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26679-1-AP (PARL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 26679-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26679-1-AP (PARL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 26679-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26679-1-AP (PARL Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human prostate cancer using 26679-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26679-1-AP (PARL Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 26679-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26679-1-AP (PARL Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 26679-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26679-1-AP (PARL Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: 3T3-L1 cells, HEK-293 cells, HeLa cells, Neuro-2a cells
• Positive IHC detected in: human prostate cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 1 publications below

Product Information

26679-1-AP targets PARL in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PARL fusion protein Ag24789
• Full Name: presenilin associated, rhomboid-like
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 36-42 kDa
• GenBank Accession Number: BC014058
• Gene Symbol: PARL
• Gene ID (NCBI): 55486
• RRID: AB_2880599
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PARL (Presenilin associated rhomboid like), is a member of the rhomboid family of intramembrane serine proteases that is localized to the inner mitochondrial membrane. PARL has 2 isoforms produced by alternative splicing with the molecular mass of 42 kDa and 37 kDa. PARL regulates mitochondrial remodeling and apoptosis through regulated substrate proteolysis. Proteolytic processing of PARL may result in the release of a small peptide, P-beta, which may transit to the nucleus. The self-regulated cleavage of PARL at positions 52-53 (α-site) and 77--78 (β-site) produce two cleaved forms of PARL named MAMP and PACT with the molecular mass of 36 kDa and 33 kDa respectively (PMID: 14732705, 28178523).

Publications

Species	Application	Title
human	WB	Mol Cell; Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria; Authors - Sabine Poerschke
human		Cell Rep; TIM23 facilitates PINK1 activation by safeguarding against OMA1-mediated degradation in damaged mitochondria; Authors - Shiori Akabane
human	WB	J Cell Biol; OMA1 protease eliminates arrested protein import intermediates upon mitochondrial depolarization; Authors - Magda Krakowczyk
human,mouse	WB	Int J Mol Sci; The Mitochondrial PHB2/OMA1/DELE1 Pathway Cooperates with Endoplasmic Reticulum Stress to Facilitate the Response to Chemotherapeutics in Ovarian Cancer.; Authors - Meiyu Cheng
human	WB	Cells; Disruption of Mitophagy Flux through the PARL-PINK1 Pathway by CHCHD10 Mutations or CHCHD10 Depletion; Authors - Tian Liu
human	WB	J Biol Chem; Diverse mitochondrial abnormalities in a new cellular model of TAFFAZZIN deficiency are remediated by cardiolipin-interacting small molecules.; Authors - Arianna F Anzmann