PARN Polyclonal antibody

PARN Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13799-1-AP

Synonyms

DAN, Deadenylating nuclease, Deadenylation nuclease, PARN



Tested Applications

Positive WB detected inHepG2 cells, human cerebellum tissue, Jurkat cell, MDA-MB-453s cells
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13799-1-AP targets PARN in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PARN fusion protein Ag4774
Full Name poly(A)-specific ribonuclease (deadenylation nuclease)
Calculated Molecular Weight 639 aa, 73 kDa
Observed Molecular Weight 74 kDa
GenBank Accession NumberBC050029
Gene Symbol PARN
Gene ID (NCBI) 5073
RRIDAB_2160430
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PARN antibody 13799-1-APDownload protocol
IHC protocol for PARN antibody 13799-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Hum Mol Genet

CGG repeats in RNA modulate expression of TDP-43 in mouse and fly models of fragile X tremor ataxia syndrome.

Authors - Jocelyn N Galloway
humanIF

Neurobiol Dis

Distinct Poly(A) nucleases have differential impact on sut-2 dependent tauopathy phenotypes.

Authors - Rebecca L Kow
mouseWB

Cell Rep

YTHDF2/3 Are Required for Somatic Reprogramming through Different RNA Deadenylation Pathways.

Authors - Jiadong Liu
humanWB

Nucleic Acids Res

Molecular mechanism of human ISG20L2 for the ITS1 cleavage in the processing of 18S precursor ribosomal RNA

Authors - Yinliang Ma