PARP10 Recombinant antibody

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  WB analysis using 86096-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86096-1-RR (PARP10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

PARP10 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86096-1-RR

Clone No.250454D4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Synonyms

ADP-ribosyltransferase diphtheria toxin-like 10, ARTD10, EC:2.4.2.-, PARP 10, PARP-10

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, MDA-MB-231 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86096-1-RR targets PARP10 in applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PARP10 fusion protein Ag23457
• Full Name: poly (ADP-ribose) polymerase family, member 10
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC136542
• Gene Symbol: PARP10
• Gene ID (NCBI): 84875
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q53GL7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PARP10 (Poly(ADP-ribose) polymerase family member 10) is a mono-ADP-ribosyltransferase that belongs to the PARP family. It regulates a variety of cellular functions, including gene transcription, DNA repair, and cell cycle control, by catalyzing ADP-ribosylation reactions. PARP10 is distributed in both the nucleus and cytoplasm, participating in the cellular response to DNA damage. In particular, its expression level significantly increases in response to UV-induced DNA damage. Additionally, PARP10 plays a crucial role in the antiviral response, being highly upregulated by interferon (IFN) to inhibit the replication of certain viruses and block protein translation during viral replication. PARP10 is also involved in immune regulation, interacting with NEMO to block the NF-κB signaling pathway and the production of pro-inflammatory cytokines.