PARP2 Polyclonal antibody

PARP2 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 55149-1-AP

Synonyms

ADPRT 2, ADPRT2, ADPRTL2, ADPRTL3, hPARP 2, pADPRT 2, PARP 2, PARP2, poly (ADP ribose) polymerase 2, Poly [ADP ribose] polymerase 2, Poly[ADP ribose] synthase 2

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Validation Data Gallery

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  WB analysis using 55149-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55149-1-AP (PARP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 55149-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 55149-1-AP (PARP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, U2OS cells, SH-SY5Y cells
• Positive IF detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

55149-1-AP targets PARP2 in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: poly (ADP-ribose) polymerase 2
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: NM_005484
• Gene Symbol: PARP2
• Gene ID (NCBI): 10038
• RRID: AB_10858796
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PARP2, also named as ADPRT2 and ADPRTL2, is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. This antibody is specific to PARP2 (N-terminal).

Publications

Species	Application	Title
human	WB	Oncogene; CPSF4 regulates circRNA formation and microRNA mediated gene silencing in hepatocellular carcinoma.; Authors - Xueying Wang
	WB	Cells; Poly(ADP-Ribose) Polymerase-1 Lacking Enzymatic Activity Is Not Compatible with Mouse Development; Authors - Tatiana Kamaletdinova
human	WB	Eur J Med Chem; Selective degradation of PARP2 by PROTACs via recruiting DCAF16 for triple-negative breast cancer.; Authors - Chunlan Pu
mouse	WB	Int J Biol Sci; PARP1 Promotes Heart Regeneration and Cardiomyocyte Proliferation; Authors - Jiangcheng Shu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hasan (Verified Customer) (12-07-2022)

WB analysis for U2OS cells (WT and PARP2KO) with PARP2 Ab (55149-1-AP) at dilution of 1:2000 overnight.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: U2OS

PARP2 Antibody Western Blot validation (1:2000 dilution) in U2OS (Cat no:55149-1-AP)