PARVB Polyclonal antibody

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• PARVB Polyclonal antibody (14463-1-AP)

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• KD/KO Validated

PARVB Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14463-1-AP Copied!

Publications (10)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA
For FC, select conjugated version.

Synonyms

β-parvin, Affixin, Beta parvin, Beta-parvin, CGI 56

• 

  WB analysis using 14463-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14463-1-AP (PARVB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse spleen using 14463-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14463-1-AP (PARVB antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach using 14463-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14463-1-AP (PARVB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 14463-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PARVB antibody (14463-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 14463-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PARVB antibody (14463-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, mouse spleen tissue, mouse heart tissue, rat heart tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14463-1-AP targets PARVB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5760

  Product name: Recombinant human PARVB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-364 aa of BC046103

  Sequence: MSSAPRSPTPRPRRMKKDESFLGKLGGTLARKRRAREVSDLQEEGKNAINSPMSPALADVHPEDTQLEENEERTMIDPTSKEDPKFKELVKVLLDWINDVLVEERIIVKQLEEDLYDGQVLQKLLEKLAGCKLNVAEVTQSEIGQKQKLQTVLEAVHDLLRPRGWALRWSVDSIHGKNLVAILHLLVSLAMHFRAPIRLPEHVTVQVVVVRKREGLLHSSHISEELTTTTEMMMGRFERDAFDTLFDHAPDKLSVVKKSLITFVNKHLNKLNLEVTELETQFADGVYLVLLMGLLEDYFVPLHHFYLTPESFDQKVHNVSFAFELMLDGGLKKPKARPEDVVNLDLKSTLRVLYNLFTKYKNVE

• Full Name: parvin, beta
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa, 45 kDa
• GenBank Accession Number: BC046103
• Gene Symbol: PARVB
• Gene ID (NCBI): 29780
• RRID: AB_2158952
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HBI1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The anti-ILK antibody (Cat# 14463-1-AP) has 10 total citations published in Nature, Cell Stem Cell, Cell Reports, Cell Death Discovery, Oncogene, Clinical Epigenetics, Scientific Reports, Journal of Biological Chemistry, and Journal of Cellular Physiology. Research fields span cancer metastasis, stem cell biology, rheumatoid arthritis, diabetic retinopathy, mitotic spindle orientation, glioblastoma prognosis, kidney development, and mammary epithelial cell differentiation.

Automatically generated from the 10 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nature; DNA of neutrophil extracellular traps promotes cancer metastasis via CCDC25.; Authors - Linbin Yang
human	WB	Cell Stem Cell; Integrin-Linked Kinase Mediates Therapeutic Resistance of Quiescent CML Stem Cells to Tyrosine Kinase Inhibitors.; Authors - Katharina Rothe
mouse	WB,IF	Cell Death Discov; Inhibition of the ILK-AKT pathway by upregulation of PARVB contributes to the cochlear cell death in Fascin2 gene knockout mice; Authors - Rongrong Liu
human	WB	Oncogene; A critical role of integrin-linked kinase, ch-TOG and TACC3 in centrosome clustering in cancer cells.; Authors - Fielding A B AB ; KD Validated
		Cell Rep; Dissociation of CCDC25 dimer into NETs-DNA-bound monomer licenses inflammatory activation of fibroblast-like synoviocytes in rheumatoid arthritis.; Authors - Yuchen Zhao
human	WB	Clin Epigenetics; TET2-mediated ECM1 hypomethylation promotes the neovascularization in active proliferative diabetic retinopathy; Authors - Chunyang Cai