PASK Polyclonal antibody

• Featured Product
• KD/KO Validated

PASK Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14396-1-AP

Synonyms

hPASK, KIAA0135, PAS kinase, PASK, PASKIN, STK37

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Validation Data Gallery

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  WB analysis of HEK-293 using 14396-1-AP

  WB result of PASK antibody (14396-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-PASK transfected HEK-293 cells.

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  WB analysis using 14396-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14396-1-AP (PASK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 14396-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14396-1-AP (PASK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 14396-1-AP

  IP result of anti-PASK (IP:14396-1-AP, 4ug; Detection:14396-1-AP 1:300) with K-562 cells lysate 4000 ug.

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  IHC staining of mouse testis using 14396-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14396-1-AP (PASK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 14396-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14396-1-AP (PASK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate hyperplasia using 14396-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 14396-1-AP (PASK Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 14396-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 14396-1-AP (PASK Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 14396-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14396-1-AP (PASK Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14396-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14396-1-AP (PASK Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 14396-1-AP

  Immunofluorescent analysis of HeLa cells using 14396-1-AP (PASK antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, K-562 cells, Jurkat cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: mouse testis tissue, human prostate hyperplasia tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14396-1-AP targets PASK in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PASK fusion protein Ag5893
• Full Name: PAS domain containing serine/threonine kinase
• Calculated Molecular Weight: 143 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: BC063585
• Gene Symbol: PASK
• Gene ID (NCBI): 23178
• RRID: AB_2878053
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAS (Per-Arnt-Sim) domains regulate the function of many intracellular signaling pathways in response to both extrinsic and intrinsic stimuli. PAS domain-containing serine/threonine-protein kinase (PASK, also termed PAS-kinase or PASKIN) is an evolutionarily conserved nutrient responsive protein kinase. PASK links energy flux and protein synthesis in yeast and regulates glycogen synthesis in mammals. It has also been implicated in glucose-stimulated INS production in pancreatic beta-cells.