CPB1 Polyclonal antibody

CPB1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12600-1-AP

Synonyms

Carboxypeptidase B, carboxypeptidase B1 (tissue), CPB, CPB1, Pancreas specific protein, PASP, PCPB

---

Validation Data Gallery

• 

  WB analysis of mouse pancreas using 12600-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12600-1-AP (CPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 12600-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12600-1-AP (CPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 12600-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12600-1-AP (CPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat pancreas using 12600-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 12600-1-AP (CPB1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12600-1-AP (CPB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12600-1-AP (CPB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12600-1-AP (CPB1 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12600-1-AP (CPB1 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12600-1-AP (CPB1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12600-1-AP (CPB1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12600-1-AP (CPB1 antibody at dilution of 1:50.

• 

  IHC staining of human pancreas using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12600-1-AP (CPB1 antibody at dilution of 1:50.

• 

  IHC staining of human pancreas using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12600-1-AP (CPB1 antibody at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12600-1-AP (CPB1 antibody at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pancreas using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 12600-1-AP (CPB1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 12600-1-AP (CPB1 antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: mouse pancreas tissue, rat pancreas tissue
• Positive IHC detected in: human pancreas tissue, human kidney tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

12600-1-AP targets CPB1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CPB1 fusion protein Ag3243
• Full Name: carboxypeptidase B1 (tissue)
• Calculated Molecular Weight: 417 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC015338
• Gene Symbol: CPB1
• Gene ID (NCBI): 1360
• RRID: AB_2261100
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PASP(pancreas-specific protein) is also named as CPB1, CPB, PCPB and belongs to the peptidase M14 family. This protein modulates the plasminogen system both in the matrix and on the cell surface, thus leading to the inhibition of endothelial cell movement and tube formation. It is initially described as a pancreatic metallocarboxypeptidase and is a marker for acute pancreatitis(PMID:19717511). The deduced 416-amino acid preproenzyme has a 15-amino acid signal peptide and a 95-amino acid activation peptide.

Publications

Species	Application	Title
human	IHC	Cancers (Basel); Is Carboxypeptidase B1 a Prognostic Marker for Ductal Carcinoma In Situ?; Authors - Charu Kothari
human	WB,IHC	Transl Oncol; Label-Free Quantitative Proteomics Unravels Carboxypeptidases as the Novel Biomarker in Pancreatic Ductal Adenocarcinoma.; Authors - Yang Song
rat	WB	Arch Gynecol Obstet; Oviductus Ranae protein hydrolyzate prevents menopausal osteoporosis by regulating TGFβ/BMP2 signaling.; Authors - Xiaohua Li