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PAX4 Polyclonal antibody

PAX4 Polyclonal Antibody for IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, ELISA

Conjugate

Unconjugated

Cat no : 13018-2-AP

Synonyms

KPD, MODY9, paired box 4, Paired box protein Pax 4, PAX4

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using PAX4 Polyclonal antibody (13018-2-AP)

    IF Staining of A549 using 13018-2-AP

    Immunofluorescent analysis of A549 cells, using PAX4 antibody 13018-2-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13018-2-AP targets PAX4 in IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAX4 fusion protein Ag4111
Full Name paired box 4
Calculated Molecular Weight 343 aa, 37 kDa
GenBank Accession NumberBC074761
Gene Symbol PAX4
Gene ID (NCBI) 5078
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAX4, a paired-box transcription factor, is a key regulator of pancreatic islet cell growth and differentiation. Differentiation of endoderm-derived endocrine pancreas is mediated through PAX4 and PAX6. PAX4 may act as a PAX6 repressor due to the competition for binding sites and lower transactivation potential of Pax-4. PAX4 has the potential to function as a tumor suppressor in human melanoma.