Tested Applications
| Positive WB detected in | Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:5000-1:20000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12563-1-AP targets PBRM1 in WB, IHC, IP, Dot blot, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3148 Product name: Recombinant human PBRM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-306 aa of BC015323 Sequence: MGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENV Predict reactive species |
| Full Name | polybromo 1 |
| Calculated Molecular Weight | 180 kDa |
| Observed Molecular Weight | 180-195 kDa, 100 kDa |
| GenBank Accession Number | BC015323 |
| Gene Symbol | PBRM1 |
| Gene ID (NCBI) | 55193 |
| RRID | AB_2877865 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86U86 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PBRM1, also named hPB1 BAF180 and Polybromo-1D, acts as a negative regulator of cell proliferation. PBRM1 is the second major mutated clear cell RCC cancer gene after VHL. PBRM1 loss is associated with low grade features.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PBRM1 antibody 12563-1-AP | Download protocol |
| IP protocol for PBRM1 antibody 12563-1-AP | Download protocol |
| WB protocol for PBRM1 antibody 12563-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PBRM1 Polyclonal antibody (Cat# 12563-1-AP) has 6 total citations published in Molecular Cell, Cell Death & Disease, Cell Communication & Signaling, Journal of the American Heart Association, Breast Cancer, and Protein Expression and Purification. Research fields span chromatin biology, renal cell carcinoma, head and neck squamous cell carcinoma, cardiomyocyte apoptosis, breast cancer angiogenesis, and protein expression.
| Species | Application | Title |
|---|---|---|
Mol Cell A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis | ||
Cell Death Dis The RBPJ/DAPK3/UBE3A signaling axis induces PBRM1 degradation to modulate the sensitivity of renal cell carcinoma to CDK4/6 inhibitors. | ||
Cell Commun Signal A positive feedback loop between BACH1 and IL-1β promotes the progression of HPV-negative head and neck squamous cell carcinoma. | ||
J Am Heart Assoc Aging-Associated ALKBH5-m6A Modification Exacerbates Doxorubicin-Induced Cardiomyocyte Apoptosis Via AT-Rich Interaction Domain 2 | ||
Protein Expr Purif Heterologous expression of the human polybromo-1 protein in the methylotrophic yeast Pichia pastoris. |
