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PC Monoclonal antibody

PC Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat , mouse and More (1)

Applications

WB, RIP, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

4F5D1

Cat no : 66615-1-Ig

Synonyms

PC, PCB, pyruvate carboxykinase, pyruvate carboxylase, Pyruvic carboxylase



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HSC-T6 cells
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

66615-1-Ig targets PC in WB, RIP, IHC,ELISA applications and shows reactivity with Human, Rat , mouse samples.

Tested Reactivity Human, Rat , mouse
Cited Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PC fusion protein Ag8846
Full Name pyruvate carboxylase
Calculated molecular weight 1178 aa, 130 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC011617
Gene symbol PC
Gene ID (NCBI) 5091
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PC antibody 66615-1-IgDownload protocol
IHC protocol for PC antibody 66615-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,RIP

Front Oncol

piR-hsa-211106 Inhibits the Progression of Lung Adenocarcinoma Through Pyruvate Carboxylase and Enhances Chemotherapy Sensitivity.

Authors - Yongmei Liu