PCCB Polyclonal antibody

PCCB Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11139-1-AP

Synonyms

PCCase subunit beta, PCCB

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Validation Data Gallery

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  WB analysis using 11139-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11139-1-AP (PCCB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 11139-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11139-1-AP (PCCB Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 11139-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11139-1-AP (PCCB Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 11139-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11139-1-AP (PCCB Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 11139-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11139-1-AP (PCCB Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of human kidney using 11139-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using 11139-1-AP (PCCB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart tissue
• Positive IHC detected in: human kidney tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

11139-1-AP targets PCCB in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCCB fusion protein Ag1608
• Full Name: propionyl Coenzyme A carboxylase, beta polypeptide
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC013768
• Gene Symbol: PCCB
• Gene ID (NCBI): 5096
• RRID: AB_2877746
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Eur J Immunol; YY1 control of mitochondrial-related genes does not account for regulation of immunoglobulin class switch recombination in mice.; Authors - Satabdi Nandi
human	WB	Biochim Biophys Acta Mol Basis Dis; ACSS3 regulates the metabolic homeostasis of epithelial cells and alleviates pulmonary fibrosis; Authors - Lan Wang

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