PCCB Polyclonal antibody

• 

  WB analysis using 11139-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11139-1-AP (PCCB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 11139-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11139-1-AP (PCCB Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 11139-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11139-1-AP (PCCB Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 11139-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11139-1-AP (PCCB Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 11139-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11139-1-AP (PCCB Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of human kidney using 11139-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using 11139-1-AP (PCCB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

PCCB Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 11139-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Propionyl-CoA carboxylase beta chain, mitochondrial, Propanoyl-CoA:carbon dioxide ligase subunit beta, PCCase subunit beta, EC:6.4.1.3

---

Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart tissue
• Positive IHC detected in: human kidney tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

11139-1-AP targets PCCB in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1608

  Product name: Recombinant human PCCB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 240-539 aa of BC013768

  Sequence: TQEELGGAKTHTTMSGVAHRAFENDVDALCNLRDFFNYLPLSSQDPAPVRECHDPSDRLVPELDTIVPLESTKAYNMVDIIHSVVDEREFFEIMPNYAKNIIVGFARMNGRTVGIVGNQPKVASGCLDINSSVKGARFVRFCDAFNIPLITFVDVPGFLPGTAQEYGGIIRHGAKLLYAFAEATVPKVTVITRKAYGGAYDVMSSKHLCGDTNYAWPTAEIAVMGAKGAVEIIFKGHENVEAAQAEYIEKFANPFPAAVRGFVDDIIQPSSTRARICCDLDVLASKKVQRPWRKHANIPL

• Full Name: propionyl Coenzyme A carboxylase, beta polypeptide
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC013768
• Gene Symbol: PCCB
• Gene ID (NCBI): 5096
• RRID: AB_2877746
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P05166
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Eur J Immunol; YY1 control of mitochondrial-related genes does not account for regulation of immunoglobulin class switch recombination in mice.; Authors - Satabdi Nandi
human	WB	Biochim Biophys Acta Mol Basis Dis; ACSS3 regulates the metabolic homeostasis of epithelial cells and alleviates pulmonary fibrosis; Authors - Lan Wang
human	IHC	Am J Pathol; Aging-associated Metabolite Methylmalonic Acid Increases Susceptibility to Pulmonary Fibrosis; Authors - Kai Xu
	WB,IF	Proc Natl Acad Sci U S A; Multifunctional biomolecular corona-inspired nanoremediation of antibiotic residues; Authors - Jie Hou
human	WB	Cancer Lett; PPDPF Promotes Esophageal Squamous Cell Carcinoma Progression by Blocking PCCA Binding to PCCB and Inhibiting Methionine Catabolism; Authors - Mengwei Li