PCDH1 Polyclonal antibody

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• PCDH1 Polyclonal antibody (13645-1-AP)

PCDH1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13645-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

Cadherin-like protein 1, PC42, Protocadherin-1, Protocadherin-42

• 

  WB analysis using 13645-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13645-1-AP (PCDH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach using 13645-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13645-1-AP (PCDH1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: fetal human brain tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13645-1-AP targets PCDH1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4591

  Product name: Recombinant human PCDH1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 61-381 aa of BC035812

  Sequence: VYKVPEEQPPNTLIGSLAADYGFPDVGHLYKLEVGAPYLRVDGKTGDIFTTETSIDREGLRECQNQLPGDPCILEFEVSITDLVQNGSPRLLEGQIEVQDINDNTPNFASPVITLAIPENTNIGSLFPIPLASDRDAGPNGVASYELQAGPEAQELFGLQVAEDQEEKQPQLIVMGNLDRERWDSYDLTIKVQDGGSPPRASSALLRVTVLDTNDNAPKFERPSYEAELSENSPIGHSVIQVKANDSDQGANAEIEYTFHQAPEVVRRLLRLDRNTGLITVQGPVDREDLSTLRFSVLAKDRGTNPKSARAQVVVTVKDMN

• Full Name: protocadherin 1
• Calculated Molecular Weight: 1060 aa, 115 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC035812
• Gene Symbol: PCDH1
• Gene ID (NCBI): 5097
• RRID: AB_2283373
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q08174
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The PCDH1 Polyclonal antibody (Cat# 13645-1-AP) has 2 citations. One appears in Cell (IF 45.1), studying human pre-implantation development, and the other in Nanomedicine (IF 4.3), investigating VEGF-mediated angiogenesis in cerebral hypoperfusion-induced cognitive dysfunction in rats. The associated research fields are developmental biology and neuroscience.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Cell; Two distinct causes contribute to the low efficiency of human pre-implantation development.; Authors - Zixuan Li
rat	WB	Nanomedicine; VEGF loaded nanofiber membranes inhibit chronic cerebral hypoperfusion-induced cognitive dysfunction by promoting HIF-1a/VEGF mediated angiogenesis; Authors - Yi-Fang Wu