PCDH11X Polyclonal antibody

PCDH11X Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20070-1-AP

Synonyms

PCDHY, PCDH-X, PCDHX, PCDH11, PCDH X

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Validation Data Gallery

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  WB analysis of human brain using 20070-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20070-1-AP (PCDH11X antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 20070-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20070-1-AP (PCDH11X antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 20070-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20070-1-AP (PCDH11X antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 20070-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20070-1-AP (PCDH11X antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 20070-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20070-1-AP (PCDH11X antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 20070-1-AP

  Immunofluorescent analysis of HepG2 cells, using PCDH11X antibody 20070-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IHC detected in: human gliomas tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20070-1-AP targets PCDH11X in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: protocadherin 11 X-linked
• Calculated Molecular Weight: 148 kDa
• Observed Molecular Weight: 135 kDa
• GenBank Accession Number: NM_032968
• Gene Symbol: PCDH11X
• Gene ID (NCBI): 27328
• RRID: AB_10667457
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PCDH11X, also named as KIAA1326, PCDH11 and PCDHX, is a potential calcium-dependent cell-adhesion protein. The antibody recognizes isoforms1 and isoform6 of PCDH11X. The antibody has no cross reaction to PCDH11Y.