PCDH15 Polyclonal antibody, PBS Only

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• PCDH15 Polyclonal antibody, PBS Only (20719-1-PBS)

• 

  WB analysis using 20719-1-AP (same clone as 20719-1-PBS)

  mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 20719-1-AP (PCDH15 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20719-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 20719-1-AP (same clone as 20719-1-PBS)

  mouse liver tissue lysates were subjected to SDS PAGE followed by western blot with 20719-1-AP (PCDH15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20719-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 20719-1-AP (same clone as 20719-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PCDH15 antibody (20719-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20719-1-PBS in a different storage buffer formulation.

PCDH15 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 20719-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

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Product Information

20719-1-PBS targets PCDH15 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: protocadherin 15
• Calculated Molecular Weight: 216 kDa
• Observed Molecular Weight: 120-160 kDa, 50 kDa
• GenBank Accession Number: NM_033056
• Gene Symbol: PCDH15
• Gene ID (NCBI): 65217
• RRID: AB_3085617
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96QU1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Protocadherin-15 (PCDH15) belongs to the cadherin superfamily that mediates calcium-dependent cell-cell adhesion and plays an important role in the maintenance of normal tissue architecture. A homozygous variant in PCDH15 results in Usher syndrome type 1F (USH1F) which leads to congenital hearing loss and syndromicretinitis pigmentosa (PMID: 38014037). Multiple isoforms of PCDH15 have been described, including 216-250 kDa (full-length), 150-180 kDa, 100-120 kDa, 50-65 kDa, and 25-40 kDa (PMID: 22363448; 15928608; 14570705; 23035094; 28883094).