CDHR2 Polyclonal antibody

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• CDHR2 Polyclonal antibody (27103-1-AP)

CDHR2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27103-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

PCDH24, Cadherin-related family member 2, PC LKC, PCLKC, PC-LKC

• 

  WB analysis of mouse liver using 27103-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 27103-1-AP (CDHR2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 27103-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27103-1-AP (CDHR2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 27103-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27103-1-AP (CDHR2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse small intestine using 27103-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 27103-1-AP (CDHR2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse small intestine using 27103-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 27103-1-AP (CDHR2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 27103-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27103-1-AP (CDHR2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 27103-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27103-1-AP (CDHR2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human small intestine using 27103-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 27103-1-AP (CDHR2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human small intestine using 27103-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 27103-1-AP (CDHR2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IHC detected in: human kidney tissue, human liver tissue, human small intestine tissue, mouse small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27103-1-AP targets CDHR2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25840

  Product name: Recombinant human PCDH24 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 381-680 aa of BC130282

  Sequence: SPRIPIDDLTMVVYDPDKGSNGTFLLSLGGPDAEAFSVSPERAAGSASVQVLVRVSALVDYERQTAMAVQVVATDSVSQNFSVAMVTIHLRDINDHRPTFPQSLYVLTVPEHSATGSVVTDSIHATDPDTGAWGQITYSLLPGNGADLFQVDPVSGTVTVRNGELLDRESQAVYYLTLQATDGGNLSSSTTLQIHLLDINDNAPVVSGSYNIFVQEEEGNVSVTIQAHDNDEPGTNNSRLLFNLLPGPYSHNFSLDPDTGLLRNLGPLDREAIDPALEGRIVLTVLVSDCGEPVLGTKVN

• Full Name: protocadherin 24
• Calculated Molecular Weight: 1310 aa, 142 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC130282
• Gene Symbol: PCDH24
• Gene ID (NCBI): 54825
• RRID: AB_2880757
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BYE9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protocadherin-24 (PCDH24, also known as CDHR2 and protocadherin LKC) was originally identified as a novel member of the human non-classical cadherin family. CDHR2 may play a role in cell-cell adhesion and contact inhibition in epithelial cells. CDHR2 expression is markedly reduced in cancers derived from liver and colon tissues, indicating that CDHR2 may play a role as a tumor suppressor protein (PMID: 23115611). The calculated molecular weight of CDHR2 is 142 kDa.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Transl Androl Urol; Discovery of bladder cancer biomarkers in paired pre- and postoperative urine samples.; Authors - Xuechao Li
		Cancer Lett; Tumor-derived CDHR2-enriched extracellular vesicles engage ANPEP on liver sinusoidal endothelial cells to facilitate transendothelial migration in PDAC.; Authors - Rui Wang