CDHR2 Polyclonal antibody

CDHR2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27103-1-AP

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

PCDH24, Cadherin-related family member 2, PC LKC, PCLKC, PC-LKC

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis using 27103-1-AP
Human urine sample was subjected to SDS PAGE followed by western blot with 27103-1-AP (CDHR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 27103-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27103-1-AP (CDHR2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 27103-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27103-1-AP (CDHR2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse small intestine using 27103-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 27103-1-AP (CDHR2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse small intestine using 27103-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 27103-1-AP (CDHR2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 27103-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27103-1-AP (CDHR2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 27103-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27103-1-AP (CDHR2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human small intestine using 27103-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 27103-1-AP (CDHR2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human small intestine using 27103-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 27103-1-AP (CDHR2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	human urine sample
Positive IHC detected in	human kidney tissue, human liver tissue, human small intestine tissue, mouse small intestine tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27103-1-AP targets CDHR2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag25840 Product name: Recombinant human PCDH24 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 381-680 aa of BC130282 Sequence: SPRIPIDDLTMVVYDPDKGSNGTFLLSLGGPDAEAFSVSPERAAGSASVQVLVRVSALVDYERQTAMAVQVVATDSVSQNFSVAMVTIHLRDINDHRPTFPQSLYVLTVPEHSATGSVVTDSIHATDPDTGAWGQITYSLLPGNGADLFQVDPVSGTVTVRNGELLDRESQAVYYLTLQATDGGNLSSSTTLQIHLLDINDNAPVVSGSYNIFVQEEEGNVSVTIQAHDNDEPGTNNSRLLFNLLPGPYSHNFSLDPDTGLLRNLGPLDREAIDPALEGRIVLTVLVSDCGEPVLGTKVN Predict reactive species
Full Name	protocadherin 24
Calculated Molecular Weight	1310 aa, 142 kDa
Observed Molecular Weight	180-200 kDa
GenBank Accession Number	BC130282
Gene Symbol	PCDH24
Gene ID (NCBI)	54825
RRID	AB_2880757
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BYE9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protocadherin-24 (PCDH24, also known as CDHR2 and protocadherin LKC) was originally identified as a novel member of the human non-classical cadherin family. CDHR2 may play a role in cell-cell adhesion and contact inhibition in epithelial cells. CDHR2 expression is markedly reduced in cancers derived from liver and colon tissues, indicating that CDHR2 may play a role as a tumor suppressor protein (PMID: 23115611).

Protocols

Product Specific Protocols
IHC protocol for CDHR2 antibody 27103-1-AP	Download protocol
WB protocol for CDHR2 antibody 27103-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The CDHR2 Polyclonal antibody (Cat# 27103-1-AP) has 2 total citations. These appear in Cancer Letters (2026), studying tumor-derived extracellular vesicles in pancreatic ductal adenocarcinoma, and Translational Andrology and Urology (2021), focused on bladder cancer biomarker discovery. The associated research fields are cancer biology, specifically pancreatic and bladder oncology.

Species	Application	Title
		Cancer Lett Tumor-derived CDHR2-enriched extracellular vesicles engage ANPEP on liver sinusoidal endothelial cells to facilitate transendothelial migration in PDAC. Authors - Rui Wang View Article
human	WB,IHC	Transl Androl Urol Discovery of bladder cancer biomarkers in paired pre- and postoperative urine samples. Authors - Xuechao Li View Article