PCDH8 Polyclonal antibody

• 

  WB analysis using 14000-1-AP

  mouse brain and unboiled mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 14000-1-AP (PCDH8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14000-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14000-1-AP (PCDH8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

PCDH8 Polyclonal Antibody for WB, ELISA

Cat No. 14000-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Protocadherin-8, Arcadlin

---

Tested Applications

• Positive WB detected in: mouse brain tissue, L02 cells, Neuro-2a cells, U-251 cells, U-87 MG cells, unboiled mouse brain

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14000-1-AP targets PCDH8 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5090

  Product name: Recombinant human PCDH8 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 31-351 aa of BC036025

  Sequence: TVRYSTFEEDAPGTVIGTLAEDLHMKVSGDTSFRLMKQFNSSLLRVREGDGQLTVGDAGLDRERLCGQAPQCVLAFDVVSFSQEQFRLVHVEVEVRDVNDHAPRFPRAQIPVEVSEGAAVGTRIPLEVPVDEDVGANGLQTVRLAEPHSPFRVELQTRADGAQCADLVLLQELDRESQAAYSLELVAQDGGRPPRSATAALSVRVLDANDHSPAFPQGAVAEVELAEDAPVGSLLLDLDAADPDEGPNGDVVFAFGARTPPEARRLFRLDPRSGRLTLAGPVDYERQDTYELDVRAQDRGPGPRAATCKVIVRIRDVNDNA

• Full Name: protocadherin 8
• Calculated Molecular Weight: 1070 aa, 113 kDa
• Observed Molecular Weight: 104 kDa,113 kDa
• GenBank Accession Number: BC036025
• Gene Symbol: PCDH8
• Gene ID (NCBI): 5100
• RRID: AB_3669175
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95206
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protocadherin-8 (PCDH8) is a member of the protocadherin family that belongs to the cadherin superfamily. Protocadherins are calcium-dependent adhesion proteins and have been implicated in neural cell-cell interactions. They are abundantly expressed in the central nervous system during embryonic development and in adulthood (PMID: 24214103). PCDH8 is a tumor suppressor involved in tumorigenesis.