PCDH9 Polyclonal antibody

PCDH9 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 25090-1-AP

Synonyms

PCDH9, protocadherin 9

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Validation Data Gallery

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  WB analysis of mouse brain using 25090-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25090-1-AP (PCDH9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 25090-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25090-1-AP (PCDH9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 25090-1-AP

  IP result of anti-PCDH9 (IP:25090-1-AP, 4ug; Detection:25090-1-AP 1:1000) with mouse brain tissue lysate 5000ug.

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  IHC staining of human gliomas using 25090-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25090-1-AP (PCDH9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 25090-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25090-1-AP (PCDH9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25090-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25090-1-AP (PCDH9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25090-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25090-1-AP (PCDH9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

25090-1-AP targets PCDH9 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCDH9 fusion protein Ag18696
• Full Name: protocadherin 9
• Calculated Molecular Weight: 1237 aa, 136 kDa
• Observed Molecular Weight: 150-180 kDa
• GenBank Accession Number: BC136626
• Gene Symbol: PCDH9
• Gene ID (NCBI): 5101
• RRID: AB_2879891
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protocadherin-9 (PCDH9) is a member of the protocadherin family that belongs to the cadherin superfamily. Protocadherins are calcium-dependent adhesion proteins and have been implicated in neural cell-cell interactions. They are abundantly expressed in the central nervous system during embryonic development and in adulthood (PMID: 24214103). PCDH9 is a single-pass type I membrane protein that contains seven cadherin motifs in its extracellular domain. It might be involved in the formation of specific neural circuits during neural development (PMID: 22982106).

What is the molecular weight of PCDH9?

The calculated molecular weight of PCDH9 is 136 kDa.

What are the isoforms of PCDH9?

Alternatively spliced transcript variants encoding distinct isoforms have been identified for the PCDH9 gene; a short transcript, a standard transcript, a transcript with additional exon 2, and another transcript with variant exon 3 (PMID: 15095963).

What is PCDH9's involvement in neural development?

The family of protocadherins is essential for neural development as mutations in these proteins give rise to neurodevelopmental disorders such as schizophrenia and epilepsy. However, the exact mechanisms of loss of function and the role of PCDH9 remain to be fully characterized (PMID: 22982106).

Publications

Species	Application	Title
mouse	WB	Neuron; Longitudinal single-cell transcriptional dynamics throughout neurodegeneration in SCA1; Authors - Leon Tejwani
human	WB	Onco Targets Ther; MiR-200a-3p promoted the malignant behaviors of ovarian cancer cells through regulating PCDH9.; Authors - Can Shi