PCDHA9 Polyclonal antibody

• Featured Product
• KD/KO Validated

PCDHA9 Polyclonal Antibody for IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 18075-1-AP

Synonyms

Protocadherin alpha-9, PCDH-alpha-9, KIAA0345

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Validation Data Gallery

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  IP experiment of mouse brain using 18075-1-AP

  IP result of anti-PCDHA9 (IP:18075-1-AP, 4ug; Detection:18075-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of rat cerebellum using 18075-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 18075-1-AP (PCDHA9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat cerebellum using 18075-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 18075-1-AP (PCDHA9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 18075-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18075-1-AP (PCDHA9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18075-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18075-1-AP (PCDHA9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 18075-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using PCDHA9 antibody (18075-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue, rat cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

18075-1-AP targets PCDHA9 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCDHA9 fusion protein Ag12767
• Full Name: protocadherin alpha 9
• Calculated Molecular Weight: 950 aa, 102 kDa
• Observed Molecular Weight: 91 kDa
• GenBank Accession Number: BC104802
• Gene Symbol: PCDHA9
• Gene ID (NCBI): 9752
• RRID: AB_2878491
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protocadherins, which constitute the largest subgroup within the cadherin superfamily, are predominantly expressed in the nervous system and are probably involved in the regulation of neuronal recognition and connectivity (PMID: 17936607; 12231349; 17133224). The protocadherin subfamily can be further subdivided into three groups: the clustered protocadherins, comprising α-, β- and γ-protocadherins; δ-protocadherins; and others, many of which are solitary (PMID: 17133224). PCDHA9 belongs to the α-protocadherin (PCDHA) cluster. A homozygous variant in PCDHA9 has been found in three unrelated Chinese ALS patients, suggesting PCDHA9 as a candidate gene for amyotrophic lateral sclerosis(PMID: 38467605).

Publications

Species	Application	Title
human	WB	Gene; Identification of two novel PCDHA9 mutations associated with Hirschsprung's disease.; Authors - Qiyang Shen ; KD Validated
human,mouse	WB,IF	Nat Commun; PCDHA9 as a candidate gene for amyotrophic lateral sclerosis; Authors - Jie Zhong