PCDHAC2 Polyclonal antibody

• 

  WB analysis using 18211-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18211-1-AP (PCDHAC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PCDHAC2 Polyclonal Antibody for WB, ELISA

Cat No. 18211-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

PCDH-alpha-C2, Protocadherin alpha-C2

---

Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18211-1-AP targets PCDHAC2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12889

  Product name: Recombinant human PCDHAC2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 42-406 aa of BC104776

  Sequence: SQLRYSVPEEQAPGALVGNVARALGLELRRLGPGCLRINHLGAPSPRYLELDLTSGALFVNERIDREALCEQRPRCLLSLEVLAHNPVAVSAVEVEILDINDNSPRFPRPNYQLQVSESVAPGARFHIESAQDPDVGANSVQTYELSPSEHFELDLKPLQENSKVLELVLRKGLDREQAALHHLVLTAVDGGIPARSGTAQISVRVLDTNDNSPAFDQSTYRVQLREDSPPGTLVVKLNASDPDEGSNGELRYSLSSYTSDRERQLFSIDASTGEVRVIGGLDYEEASSYQIYVQATDRGPVPMAGHCKVLVDIVDVNDNAPEVVLTDLYSPVPENATPNTIVAVLSVNDQDSGPNRKVSLGLEA

• Full Name: protocadherin alpha subfamily C, 2
• Calculated Molecular Weight: 884 aa, 96 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC104776
• Gene Symbol: PCDHAC2
• Gene ID (NCBI): 56134
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y5I4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protocadherins, which constitute the largest subgroup within the cadherin superfamily, are predominantly expressed in the nervous system and are probably involved in the regulation of neuronal recognition and connectivity (PMID: 17936607; 12231349; 17133224). The protocadherin subfamily can be further subdivided into three groups: the clustered protocadherins, comprising α-, β-, and γ-protocadherins; δ-protocadherins; and others, many of which are solitary (PMID: 17133224). PCDHAC2 is a subtype of α-protocadherins, mainly expressed by serotonergic neurons. PCDHAC2 is essential for the diffuse projection of serotonergic axons (PMID: 29162883).