PCDHB10 Polyclonal antibody

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  IHC staining of human pancreas cancer using 14785-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14785-1-AP (PCDHB10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 14785-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14785-1-AP (PCDHB10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 14785-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14785-1-AP (PCDHB10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 14785-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14785-1-AP (PCDHB10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PCDHB10 Polyclonal Antibody for IHC, ELISA

Cat No. 14785-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

PCDH beta 10, PCDH BETA10, PCDHB10, PCHB10, protocadherin beta 10

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Tested Applications

• Positive IHC detected in: human pancreas cancer tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14785-1-AP targets PCDHB10 in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6427

  Product name: Recombinant human PCDHB10 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 400-696 aa of BC031837

  Sequence: FYILITEGALDREIRAEYNITITVTDLGTPRLKTEHNITVLVSDVNDNAPAFTQTSYTLFVRENNSPALHIGSVSATDRDSGTNAQVTYSLLPPQDPHLPLASLVSINADNGHLFALRSLDYEALQAFEFRVGATDRGSPALSREALVRVLVLDANDNSPFVLYPLQNGSAPCTELVPRAAEPGYLVTKVVAVDGDSGQNAWLSYQLLKATEPGLFGVWAHNGEVRTARLLSERDAAKHRLVVLVKDNGEPPRSATATLHLLLVDGFSQPYLPLPEAAPAQAQAEADLLTVYLVVAL

• Full Name: protocadherin beta 10
• Calculated Molecular Weight: 88 kDa
• GenBank Accession Number: BC031837
• Gene Symbol: PCDHB10
• Gene ID (NCBI): 56126
• RRID: AB_3669193
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UN67
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protocadherins, which constitute the largest subgroup within the cadherin superfamily, are predominantly expressed in the nervous system and are probably involved in the regulation of neuronal recognition and connectivity (PMID: 17936607; 12231349; 17133224). The protocadherin subfamily can be further subdivided into three groups: the clustered protocadherins, comprising α-, β- and γ-protocadherins; δ-protocadherins; and others, many of which are solitary (PMID: 17133224). PCDHB10 belongs to the β-protocadherin (PCDHB) cluster. PCDHB cluster acts as a DNA methylation marker of aggressiveness in neuroblastoma (PMID: 26103900).