Tested Applications
| Positive WB detected in | K-562 cells, HeLa cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23540-1-AP targets PCF11 in WB, IHC, IF, IP, ELISA, CUT&Tag applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20263 Product name: Recombinant human PCF11 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1206-1555 aa of BC146778 Sequence: IPAPMTVGNIQASQQVLSGVAQPVAFGQGQQFLPVHPQNPGFVQNPSGALPKAYPDNHLSQVDVNELFSKLLKTGILKLSQTDSATTQVSEVTAQPPPEEEEDQNEDQDVPDLTNFTVEELKQRYDSVINRLYTGIQCYSCGMRFTTSQTDVYADHLDWHYRQNRTEKDVSRKVTHRRWYYSLTDWIEFEEIADLEERAKSQFFEKVHEEVVLKTQEAAKEKEFQSVPAGPAGAVESCEICQEQFEQYWDEEEEEWHLKNAIRVDGKIYHPSCYEDYQNTSSFDCTPSPSKTPVENPLNIMLNIVKNELQEPCDSPKVKEERIDTPPACTEESIATPSEIKTENDTVESV Predict reactive species |
| Full Name | PCF11, cleavage and polyadenylation factor subunit, homolog (S. cerevisiae) |
| Calculated Molecular Weight | 1555 aa, 173 kDa |
| Observed Molecular Weight | 173 kDa |
| GenBank Accession Number | BC146778 |
| Gene Symbol | PCF11 |
| Gene ID (NCBI) | 51585 |
| RRID | AB_2879293 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | O94913 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
In Saccharomyces cerevisiae, the cleavage/polyadenylation factor Pcf11 is a crucial regulatory factor required for recruiting polyadenylation machinery to elongating RNA polymerase II (RNAPII), and is necessary for correct transcriptional termination. Pcf11 (PCF11, cleavage and polyadenylation factor subunit, homolog (S. cerevisiae)), is a 1,555 amino acid nuclear protein that is a component of pre-mRNA cleavage complex II. It is suggested that Pcf11 is capable of promoting the dissociation of Pol II elongation complexes from DNA. Pcf11 contains a CTD-interaction domain that binds in a phospho-dependent manner to the heptad repeats within the RNA polymerase II CTD. The gene encoding Pcf11 is located on human chromosoem 11, which houses over 1,400 genes and comprises nearly 4% of the human genome. Jervell and Lange-Nielsen syndrome, Jacobsen syndrome, Niemann-Pick disease, hereditary angioedema and Smith-Lemli-Opitz syndrome are associated with defects in genes that maps to chromosome 11. This antibody is specific to the 173kd human PCF11 protein.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PCF11 antibody 23540-1-AP | Download protocol |
| IP protocol for PCF11 antibody 23540-1-AP | Download protocol |
| WB protocol for PCF11 antibody 23540-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PCF11 Polyclonal antibody (catalog 23540-1-AP) has 8 total citations. These appear in journals including Neuron, Molecular Cell, Nucleic Acids Research, Microbiological Research, Cell Reports, and Biochimie. Research fields span transcription regulation, pre-mRNA 3' end processing and alternative polyadenylation, neurodegeneration, cancer (lung and prostate), and swine influenza virus replication, with applications mainly in Western blot and immunofluorescence.
| Species | Application | Title |
|---|---|---|
Neuron Suppression of premature transcription termination leads to reduced mRNA isoform diversity and neurodegeneration. | ||
Mol Cell RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits | ||
Nucleic Acids Res CRISPRpas: programmable regulation of alternative polyadenylation by dCas9.
| ||
Microbiol Res Pre-mRNA cleavage complex II protein Pcf11 facilitates swine influenza virus replication by interacting with viral NP and promoting polymerase activity | ||
Cell Rep LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation | ||
Cell Rep CPSF1 inhibition promotes widespread use of intergenic polyadenylation sites and impairs glycolysis in prostate cancer cells |
Reviews
What customers say
One reviewer tested this antibody on Jurkat nuclear cell extracts by Western Blot at a 1:1000 dilution and rated it 4 out of 5. They observed two major bands: one between 170 and 235 kDa and another above 235 kDa, consistent with expected isoform sizes. Overall, the antibody performed well for detecting the target in nuclear extracts, with clear banding patterns at the recommended dilution.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Fabienne (Verified Customer) (05-09-2025) | I obtained two major bands in western blot, one between 170 and 235 KDa and another above 235 KDa. Tested on nuclear cell extracts
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