Tested Applications
| Positive IHC detected in | human breast hyperplasia tissue, human breast cancer tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
This antibody is recommended for IHC.
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26638-1-AP targets PCID2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24417 Product name: Recombinant human PCID2 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-397 aa of BC031246 Sequence: MRLTDVVQQLVYEAIDSRDGASCAELVSFKHPHVANPRLQMASPEEKCQQVLEPPYDEMFAAHLRCTYAVGDHDFIEAYKCQTVIVQSFLRAFQAHKEENWALPVMYAVALDLRVFANNADQQLVKKGKSKVGDMLEKAAELLMSCFRVCASDTRAGIEDSKKWGMLFLVNQLFKIYFKINKLHLCKPLIRAIDSSNLKDDYSTAQRVTYKYYVGRKAMFDSDFKQAEEYLSFAFEHCHRSSQKNKRMILIYLLPVKMLLGHMPTVELLKKYHLMQFAEVTRAVSEGNLLLLHEALAKHEAFFIRCGIFLILEKLKIITYRNLFKKVYLLLKTHQLSLDAFLVALKFMQVEDVDIDEVQCILANLIYMGHVKGYISHQHQKLVVSKQNPFPPLSTVC Predict reactive species |
| Full Name | PCI domain containing 2 |
| Calculated Molecular Weight | 399 aa, 46 kDa |
| GenBank Accession Number | BC031246 |
| Gene Symbol | PCID2 |
| Gene ID (NCBI) | 55795 |
| RRID | AB_2918105 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5JVF3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Human PCID2, is characterized by its PCI interaction domain and its involvement in the mammalian transcription export complex (TREX-2). PCID2 colocalizes with gamma-tubulin and centrin-2 at the centrosome of HeLa cells. It's required for B-cell survival through the regulation of the expression of cell-cycle checkpoint MAD2L1 protein during B cell differentiation. PCID2 has some isoforms with MW 43-46 kDa and 52 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PCID2 antibody 26638-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PCID2 Polyclonal antibody (Cat# 26638-1-AP) has 1 total citation. It appears in Scientific Reports (IF 4.9), published in 2025. The cited study investigates how curcumin preserves bone health in diabetes by inhibiting osteoporosis through regulation of the SIRT3/FoxO3a signaling pathway, using WB and IHC applications in mouse samples. The associated research field is osteoporosis and diabetic bone disease.
| Species | Application | Title |
|---|---|---|
Sci Rep Curcumin preserves bone health compromised by diabetes by inhibiting osteoporosis through regulation of the SIRT3/FoxO3a signalling pathway. |
