PCID2 Polyclonal antibody

PCID2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 26638-1-AP

Synonyms

CSN12 like protein, F10, PCI domain containing 2, PCID2

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Validation Data Gallery

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  IHC staining of human breast hyperplasia using 26638-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 26638-1-AP (PCID2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 26638-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26638-1-AP (PCID2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 26638-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 26638-1-AP (PCID2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human breast hyperplasia tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26638-1-AP is PCID2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCID2 fusion protein Ag24417
• Full Name: PCI domain containing 2
• Calculated Molecular Weight: 399 aa, 46 kDa
• GenBank Accession Number: BC031246
• Gene Symbol: PCID2
• Gene ID (NCBI): 55795
• RRID: AB_2918105
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human PCID2, is characterized by its PCI interaction domain and its involvement in the mammalian transcription export complex (TREX-2). PCID2 colocalizes with gamma-tubulin and centrin-2 at the centrosome of HeLa cells. It's required for B-cell survival through the regulation of the expression of cell-cycle checkpoint MAD2L1 protein during B cell differentiation. PCID2 has some isoforms with MW 43-46 kDa and 52 kDa.