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PCMT1 Polyclonal antibody

PCMT1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10519-1-AP

Synonyms

PCMT1



Tested Applications

Positive WB detected inHeLa, human testis tissue, rat testis tissue, mouse testis tissue, Raji cells, HeLa cells, HEK-293 cells
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 4 publications below

Product Information

10519-1-AP targets PCMT1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCMT1 fusion protein Ag0811
Full Name protein-L-isoaspartate (D-aspartate) O-methyltransferase
Calculated molecular weight 25 kDa
Observed molecular weight 25-28 kDa
GenBank accession numberBC008748
Gene symbol PCMT1
Gene ID (NCBI) 5110
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The PCMT1 gene encodes the protein repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase, which is known to protect certain neural cells against Bax-induced apoptosis.

Protocols

Product Specific Protocols
WB protocol for PCMT1 antibody 10519-1-APDownload protocol
IHC protocol for PCMT1 antibody 10519-1-APDownload protocol
IF protocol for PCMT1 antibody 10519-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Arch Gen Psychiatry

Common proteomic changes in the hippocampus in schizophrenia and bipolar disorder and particular evidence for involvement of cornu ammonis regions 2 and 3.

Authors - Melanie Föcking
humanWB

Front Cell Dev Biol

LINC00511/miRNA-143-3p Modulates Apoptosis and Malignant Phenotype of Bladder Carcinoma Cells via PCMT1.

Authors - Li-Ming Dong
mouseWB

Int J Cardiol

Protein-L-isoaspartate (D-aspartate) O-methyltransferase protects cardiomyocytes against hypoxia induced apoptosis through inhibiting proapoptotic kinase Mst1.

Authors - Yan Guijun G
humanWB

Diabetologia

Post-translational protein modifications in type 1 diabetes: a role for the repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase?

Authors - W?gner A M AM