PCMT1 Polyclonal antibody

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  WB analysis using 10519-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10519-1-AP (PCMT1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10519-1-AP

  WB result of PCMT1 antibody (10519-1-AP, 1:1000) with si-Control and si-PCMT1 transfected HeLa cells.

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  WB analysis of HEK-293 using 10519-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10519-1-AP (PCMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10519-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10519-1-AP (PCMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10519-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10519-1-AP (PCMT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 10519-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10519-1-AP (PCMT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 10519-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10519-1-AP (PCMT1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 10519-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10519-1-AP (PCMT1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HEK-293 using 10519-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 10519-1-AP (PCMT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

PCMT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 10519-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

L-isoaspartyl protein carboxyl methyltransferase, PCMT-1, PIMT, protein L isoaspartate (D aspartate) O methyltransferase, Protein L-isoaspartyl/D-aspartyl methyltransferase

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Tested Applications

• Positive WB detected in: HeLa, HeLa cells, human testis tissue, HEK-293 cells, Raji cells, mouse testis tissue, rat testis tissue
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 2 publications below

Product Information

10519-1-AP targets PCMT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0811

  Product name: Recombinant human PCMT1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-227 aa of BC008748

  Sequence: MAWKSGGASHSELIHNLRKNGIIKTDKVFEVMLATDRSHYAKCNPYMDSPQSIGFQATISAPHMHAYALELLFDQLHEGAKALDVGSGSGILTACFARMVGCTGKVIGIDHIKELVDDSINNVRKDDPTLLSSGRVQLVVGDGRMGYAEEAPYDAIHVGAAAPVVPQALIDQLKPGGRLILPVGPAGGNQMLEQYDKLQDGSIKMKPLMGVIYVPLTDKEKQWSRWK

• Full Name: protein-L-isoaspartate (D-aspartate) O-methyltransferase
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25-28 kDa
• GenBank Accession Number: BC008748
• Gene Symbol: PCMT1
• Gene ID (NCBI): 5110
• RRID: AB_2160201
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P22061
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The PCMT1 gene encodes the protein repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase, which is known to protect certain neural cells against Bax-induced apoptosis.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Diabetologia; Post-translational protein modifications in type 1 diabetes: a role for the repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase?; Authors - W?gner A M AM
mouse	WB	Int J Cardiol; Protein-L-isoaspartate (D-aspartate) O-methyltransferase protects cardiomyocytes against hypoxia induced apoptosis through inhibiting proapoptotic kinase Mst1.; Authors - Yan Guijun G
human	WB	Front Cell Dev Biol; LINC00511/miRNA-143-3p Modulates Apoptosis and Malignant Phenotype of Bladder Carcinoma Cells via PCMT1.; Authors - Li-Ming Dong
human	WB	Biology (Basel); In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.; Authors - Olga Gourdomichali
human	IHC	Transl Cancer Res; Elevated expression of protein-L-isoaspartate O-methyltransferase-1 (PCMT1) in cervical cancer; Authors - Liyun Shan
human	WB	J Exp Clin Cancer Res; Genome-wide CRISPR/Cas9 library screen identifies PCMT1 as a critical driver of ovarian cancer metastasis.; Authors - Jingjing Zhang ; KD Validated