PCMT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PCMT1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 10519-1-AP

Synonyms

PCMT1

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Validation Data Gallery

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  WB analysis of HeLa using 10519-1-AP

  WB result of PCMT1 antibody (10519-1-AP, 1:1000) with si-Control and si-PCMT1 transfected HeLa cells.

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  WB analysis of human testis using 10519-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10519-1-AP (PCMT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10519-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10519-1-AP (PCMT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10519-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10519-1-AP (PCMT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 10519-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 10519-1-AP (PCMT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10519-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10519-1-AP (PCMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10519-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10519-1-AP (PCMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 10519-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10519-1-AP (PCMT1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 10519-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10519-1-AP (PCMT1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 10519-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 10519-1-AP (PCMT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa, human testis tissue, rat testis tissue, mouse testis tissue, Raji cells, HeLa cells, HEK-293 cells
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below

Product Information

10519-1-AP targets PCMT1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCMT1 fusion protein Ag0811
• Full Name: protein-L-isoaspartate (D-aspartate) O-methyltransferase
• Calculated molecular weight: 25 kDa
• Observed molecular weight: 25-28 kDa
• GenBank accession number: BC008748
• Gene symbol: PCMT1
• Gene ID (NCBI): 5110
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The PCMT1 gene encodes the protein repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase, which is known to protect certain neural cells against Bax-induced apoptosis.

Publications

Species	Application	Title
human	WB	Diabetologia; Post-translational protein modifications in type 1 diabetes: a role for the repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase?; Authors - W?gner A M AM
mouse	WB	Int J Cardiol; Protein-L-isoaspartate (D-aspartate) O-methyltransferase protects cardiomyocytes against hypoxia induced apoptosis through inhibiting proapoptotic kinase Mst1.; Authors - Yan Guijun G
human	WB	Front Cell Dev Biol; LINC00511/miRNA-143-3p Modulates Apoptosis and Malignant Phenotype of Bladder Carcinoma Cells via PCMT1.; Authors - Li-Ming Dong
human	WB	Biology (Basel); In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.; Authors - Olga Gourdomichali
human	WB	J Exp Clin Cancer Res; Genome-wide CRISPR/Cas9 library screen identifies PCMT1 as a critical driver of ovarian cancer metastasis.; Authors - Jingjing Zhang ; KD Validated
mouse	WB	Arch Gen Psychiatry; Common proteomic changes in the hippocampus in schizophrenia and bipolar disorder and particular evidence for involvement of cornu ammonis regions 2 and 3.; Authors - Melanie Föcking