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PCMT1 Polyclonal antibody

PCMT1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10519-1-AP

Synonyms

PCMT1



Tested Applications

Positive WB detected inHeLa, human testis tissue, rat testis tissue, mouse testis tissue, Raji cells, HeLa cells, HEK-293 cells
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10519-1-AP targets PCMT1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCMT1 fusion protein Ag0811
Full Name protein-L-isoaspartate (D-aspartate) O-methyltransferase
Calculated molecular weight 25 kDa
Observed molecular weight 25-28 kDa
GenBank accession numberBC008748
Gene symbol PCMT1
Gene ID (NCBI) 5110
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The PCMT1 gene encodes the protein repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase, which is known to protect certain neural cells against Bax-induced apoptosis.

Protocols

Product Specific Protocols
WB protocol for PCMT1 antibody 10519-1-APDownload protocol
IHC protocol for PCMT1 antibody 10519-1-APDownload protocol
IF protocol for PCMT1 antibody 10519-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Diabetologia

Post-translational protein modifications in type 1 diabetes: a role for the repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase?

Authors - W?gner A M AM
mouseWB

Int J Cardiol

Protein-L-isoaspartate (D-aspartate) O-methyltransferase protects cardiomyocytes against hypoxia induced apoptosis through inhibiting proapoptotic kinase Mst1.

Authors - Yan Guijun G
humanWB

Front Cell Dev Biol

LINC00511/miRNA-143-3p Modulates Apoptosis and Malignant Phenotype of Bladder Carcinoma Cells via PCMT1.

Authors - Li-Ming Dong
humanWB

Biology (Basel)

In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.

Authors - Olga Gourdomichali
humanWB

J Exp Clin Cancer Res

Genome-wide CRISPR/Cas9 library screen identifies PCMT1 as a critical driver of ovarian cancer metastasis.

Authors - Jingjing Zhang
  • KD Validated
mouseWB

Arch Gen Psychiatry

Common proteomic changes in the hippocampus in schizophrenia and bipolar disorder and particular evidence for involvement of cornu ammonis regions 2 and 3.

Authors - Melanie Föcking