PCMT1 Monoclonal antibody

PCMT1 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3C8G8

Cat no : 60172-1-Ig

Synonyms

PCMT1

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Validation Data Gallery

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  WB analysis using 60172-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60172-1-Ig (PCMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 60172-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60172-1-Ig (PCMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 60172-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 60172-1-Ig (PCMT1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 60172-1-Ig

  Immunofluorescent analysis of HeLa cells using 60172-1-Ig (PCMT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, Raji cells, pig brain tissue, HEK-293 cells, HepG2 cells, K-562 cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:10000
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Product Information

60172-1-Ig targets PCMT1 in WB, IF, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PCMT1 fusion protein Ag6277
• Full Name: protein-L-isoaspartate (D-aspartate) O-methyltransferase
• Calculated molecular weight: 25 kDa
• Observed molecular weight: 25-27 kDa
• GenBank accession number: BC008748
• Gene symbol: PCMT1
• Gene ID (NCBI): 5110
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The PCMT1 gene encodes the protein repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase, which is known to protect certain neural cells against Bax-induced apoptosis.