PCP4 Polyclonal antibody

PCP4 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14705-1-AP

Synonyms

Brain specific antigen PCP 4, PCP4, PEP 19, PEP19, Purkinje cell protein 4



Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman cerebellum tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14705-1-AP targets PCP4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCP4 fusion protein Ag6388
Full Name Purkinje cell protein 4
Calculated molecular weight 7 kDa
Observed molecular weight 8 kDa
GenBank accession numberBC013791
Gene symbol PCP4
Gene ID (NCBI) 5121
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PCP4, also named as PEP19, belongs to a family of proteins involved in calcium transduction signals and binds calmodulin via an IQ motif, in a calcium independent manner. It is mainly expressed in ectoderm and neuroectoderm comprising neural crest derived cells.

Protocols

Product Specific Protocols
WB protocol for PCP4 antibody 14705-1-APDownload protocol
IHC protocol for PCP4 antibody 14705-1-APDownload protocol
IP protocol for PCP4 antibody 14705-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Nat Neurosci

Acute silencing of hippocampal CA3 reveals a dominant role in place field responses.

Authors - Heydar Davoudi
humanWB

Ann Neurol

PMID: 32167609

Authors - Jimena Monzón-Sandoval