CoraLite® Plus 488-conjugated PCP4 Polyclonal antibody

PCP4 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14705

Synonyms

Brain specific antigen PCP 4, PCP4, PEP 19, PEP19, Purkinje cell protein 4

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Validation Data Gallery

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  IF Staining of mouse brain using CL488-14705

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 PCP4 antibody (CL488-14705) at dilution of 1:200.

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  IF Staining of mouse brain using CL488-14705

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 PCP4 antibody (CL488-14705) at dilution of 1:200.

• 

  IF Staining of mouse brain using CL488-14705

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 PCP4 antibody (CL488-14705) at dilution of 1:200, ADCY3 antibody (19492-1-AP, red).

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Tested Applications

• Positive IF detected in: mouse brain tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-14705 targets PCP4 in IF applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCP4 fusion protein Ag6388
• Full Name: Purkinje cell protein 4
• Calculated Molecular Weight: 7 kDa
• Observed Molecular Weight: 8 kDa
• GenBank Accession Number: BC013791
• Gene Symbol: PCP4
• Gene ID (NCBI): 5121
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PCP4, also named as PEP19, belongs to a family of proteins involved in calcium transduction signals and binds calmodulin via an IQ motif, in a calcium independent manner. It is mainly expressed in ectoderm and neuroectoderm comprising neural crest derived cells.