PCSK2 Polyclonal antibody, PBS Only

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• PCSK2 Polyclonal antibody, PBS Only (10553-1-PBS)

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  WB analysis using 10553-1-AP (same clone as 10553-1-PBS)

  mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 10553-1-AP (PCSK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10553-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 10553-1-AP (same clone as 10553-1-PBS)

  mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 10553-1-AP (PCSK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10553-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 10553-1-AP (same clone as 10553-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10553-1-AP (PCSK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10553-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 10553-1-AP (same clone as 10553-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10553-1-AP (PCSK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10553-1-PBS in a different storage buffer formulation.

PCSK2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 10553-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

EC:3.4.21.94, KEX2-like endoprotease 2, NEC2, PC2, SPC2

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Product Information

10553-1-PBS targets PCSK2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0852

  Product name: Recombinant human PCSK2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 338-638 aa of BC005815

  Sequence: GRTALYDESCSSTLASTFSNGRKRNPEAGVATTDLYGNCTLRHSGTSAAAPEAAGVFALALEANLGLTWRDMQHLTVLTSKRNQLHDEVHQWRRNGVGLEFNHLFGYGVLDAGAMVKMAKDWKTVPERFHCVGGSVQDPEKIPSTGKLVLTLTTDACEGKENFVRYLEHVQAVITVNATRRGDLNINMTSPMGTKSILLSRRPRDDDSKVGFDKWPFMTTHTWGEDARGTWTLELGFVGSAPQKGVLKEWTLMLHGTQSAPYIDQVVRDYQSKLAMSKKEELEEELDEAVERSLKSILNKN

• Full Name: proprotein convertase subtilisin/kexin type 2
• Calculated Molecular Weight: 70.5 kDa
• Observed Molecular Weight: 65-75 kDa
• GenBank Accession Number: BC005815
• Gene Symbol: PCSK2
• Gene ID (NCBI): 5126
• RRID: AB_2299124
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P16519
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PCSK2(Prohormone convertase 2) is also named as NEC2(neuroendocrine convertase 2), PC2 and belongs to the peptidase S8 family. It is involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. PCSK2 is responsible for the release of glucagon from proglucagon in pancreatic A cells. This protein has 3 isoforms produced by alternative splicing. PCSK2 is biosynthesized as a 74-kDa inactive precursor which is matured to a 64-kDa active enzyme by autocatalytic removal of its prodomain (PMID: 8397508, 21092739)