Mouse PCSK9 Recombinant monoclonal antibody, PBS Only

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  IF Staining of mouse liver using 83004-4-RR (same clone as 83004-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using PCSK9 antibody (83004-4-RR, Clone: 230536E4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83004-4-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 83004-4-RR (same clone as 83004-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using PCSK9 antibody (83004-4-RR, Clone: 230536E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83004-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83004-4-RR (same clone as 83004-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PCSK9 antibody (83004-4-RR, Clone: 230536E4 ) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83004-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83004-4-RR (same clone as 83004-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug PCSK9 Recombinant antibody (83004-4-RR, Clone:230536E4) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83004-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83004-4-PBS

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Mouse PCSK9 Recombinant monoclonal antibody, PBS Only (83004-4-PBS, Clone:230536E4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

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  Affinity and Kinetic Characterization of 83004-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83004-4-RR against Mouse PCSK9 were performed. The affinity constant is 384 nM.

PCSK9 Uni-rAb^® Recombinant Antibody for IF/ICC, IF-P, FC (Intra), Indirect ELISA

Cat No. 83004-4-PBS

Clone No.230536E4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IF-P, FC (Intra), Indirect ELISA

Synonyms

230536E4, AI415265, AI747682, FH3, HCHOLA3

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Product Information

83004-4-PBS targets PCSK9 in IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0640

  Product name: Recombinant Mouse PCSK9 protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-10*His

  Domain: 35-694 aa of NM_153565.2

  Sequence: QDEDGDYEELMLALPSQEDGLADEAAHVATATFRRCSKEAWRLPGTYIVVLMEETQRLQIEQTAHRLQTRAARRGYVIKVLHIFYDLFPGFLVKMSSDLLGLALKLPHVEYIEEDSFVFAQSIPWNLERIIPAWHQTEEDRSPDGSSQVEVYLLDTSIQGAHREIEGRVTITDFNSVPEEDGTRFHRQASKCDSHGTHLAGVVSGRDAGVAKGTSLHSLRVLNCQGKGTVSGTLIGLEFIRKSQLIQPSGPLVVLLPLAGGYSRILNAACRHLARTGVVLVAAAGNFRDDACLYSPASAPEVITVGATNAQDQPVTLGTLGTNFGRCVDLFAPGKDIIGASSDCSTCFMSQSGTSQAAAHVAGIVARMLSREPTLTLAELRQRLIHFSTKDVINMAWFPEDQQVLTPNLVATLPPSTHETGGQLLCRTVWSAHSGPTRTATATARCAPEEELLSCSSFSRSGRRRGDWIEAIGGQQVCKALNAFGGEGVYAVARCCLVPRANCSIHNTPAARAGLETHVHCHQKDHVLTGCSFHWEVEDLSVRRQPALRSRRQPGQCVGHQAASVYASCCHAPGLECKIKEHGISGPSEQVTVACEAGWTLTGCNVLPGASLTLGAYSVDNLCVARVHDTARADRTSGEATVAAAICCRSRPSAKASWVQ

• Full Name: proprotein convertase subtilisin/kexin type 9
• Calculated Molecular Weight: 75KD
• GenBank Accession Number: NM_153565.2
• Gene Symbol: Pcsk9
• Gene ID (NCBI): 100102
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q80W65
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PCSK9 (Proprotein convertase subtilisin/kexin type 9) is the ninth member of the proprotein convertase family that regulates lipoprotein homeostasis and altered PCSK9 expression was reportedly associated with tumor development and progression. PCSK9 is primarily synthesized and expressed in the liver but also in many other tissues, such as the intestinal tract, kidney, brain, heart, and blood vessels (PMID: 36242053).