PCYT1B Polyclonal antibody

PCYT1B Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13765-1-AP

Synonyms

CCT B, CCT beta, CCTB, CT B, CTB, PCYT1B

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Validation Data Gallery

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  WB analysis of human placenta using 13765-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13765-1-AP (PCYT1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13765-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13765-1-AP (PCYT1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human placenta using 13765-1-AP

  IP result of anti-PCYT1B (IP:13765-1-AP, 4ug; Detection:13765-1-AP 1:500) with human placenta tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: human placenta tissue, human brain tissue
• Positive IP detected in: human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13765-1-AP targets PCYT1B in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCYT1B fusion protein Ag4748
• Full Name: phosphate cytidylyltransferase 1, choline, beta
• Calculated Molecular Weight: 351 aa, 40 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC045634
• Gene Symbol: PCYT1B
• Gene ID (NCBI): 9468
• RRID: AB_10642953
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Neurobiol Dis; Choline metabolism in regulating inflammatory bowel disease-linked anxiety disorders: A multi-omics exploration of the gut-brain axis; Authors - Fan Zhang