Tested Applications
| Positive WB detected in | LNCaP cells, human brain tissue |
| Positive IP detected in | human placenta tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13765-1-AP targets PCYT1B/PCYT1A in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4748 Product name: Recombinant human PCYT1B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC045634 Sequence: MVGNQECIMEEDNRAPQLWRKTLTAPAPFADETNCQCQAPHEKLTIAQARLGTPADRPVRVYADGIFDLFHSGHARALMQAKTLFPNSYLLVGVCSDDLTHKFKGFTVMNEAERYEALRHCRYVDEVIRDAPWTLTPEFLEKHKIDFVAHDDIPYSSAGSDDVYKHIKEAGMFVPTQRTEGISTSDIITRIVRDYDVYARRNLQRGYTAKELNVSFINEKRYRFQNQVDKMKEKVKNVEERSKEFVNRVEEKSHDLIQKWEEKSREFIGNFLELFGPDGAWKQMFQERSSRMLQALSPKQSPVSSPTRSRSPSRSPSPTFSWLPLKTSPPSSPKAASASISSMSEGDEDEK Predict reactive species |
| Full Name | phosphate cytidylyltransferase 1, choline, beta |
| Calculated Molecular Weight | 351 aa, 40 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC045634 |
| Gene Symbol | PCYT1B |
| Gene ID (NCBI) | 9468 |
| RRID | AB_10642953 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y5K3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PCYT1B (Phosphate Cytidylyltransferase 1B, Choline), also named as CTβ, CCTB and CCT-Beta, is encoded by the gene belongs to the cytidylyltransferase family. It is involved in the regulation of phosphatidylcholine biosynthesis. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. PCYT1B encodes three isoforms of CTβ (CTβ1, CTβ2 and CTβ3). CTβ1, CTβ2 isoforms are present in humans and CTβ2 and CTβ3 isoforms have been identified in mice. The CTβ2 can be detected as 41 kDa, and CTβ3 can be detected as 39 kDa. This antibody can recognizes both PCYT1A and PCYT1B. PCYT1A usually exists in the form of a 80 kDa dimer.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for PCYT1B/PCYT1A antibody 13765-1-AP | Download protocol |
| WB protocol for PCYT1B/PCYT1A antibody 13765-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PCYT1B/PCYT1A Polyclonal antibody (Cat# 13765-1-AP) has 1 citation. It was used in a Western Blot study published in Neurobiology of Disease (2023), investigating choline metabolism in inflammatory bowel disease-linked anxiety disorders through a multi-omics gut-brain axis exploration. The research field encompasses neurobiology, metabolic regulation, and gastrointestinal disorders.
| Species | Application | Title |
|---|---|---|
Neurobiol Dis Choline metabolism in regulating inflammatory bowel disease-linked anxiety disorders: A multi-omics exploration of the gut-brain axis |
Reviews
What customers say
The product received one review rated 4 out of 5. The reviewer used it for Western Blot at a 1:4000 dilution on mouse liver tissue and reported it as a good antibody with a strong signal. Overall, the feedback is positive, highlighting reliable performance and clear detection in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hua (Verified Customer) (10-22-2025) | Good antibody for WB. Strong signal.
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