Mouse PD-1/CD279 Recombinant monoclonal antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP00496-4

  Sandwich ELISA standard curve of MP00496-4, Mouse PD-1/CD279 Monoclonal Matched Antibody Pair - PBS only. 83516-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0918. 83516-9-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL.

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  Sample test of MP00496-4

  The mean mouse PD-1/CD279 concentration was determined to be 14.08 ng/mL in EL-4 cell extract based on a 3.0 mg/mL extract load.

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  Sample test of MP00496-4

  EL-4 was cultured in RPMI 1640 supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, and 100 μg/mL ofstreptomycin sulfate.The cellswere stimulated with 10 μg/mL of PHA and 10 ng/mL of PMA. An aliquot of the cell culture supernatant wasremoved, assayed for mouse PD-1/CD279 and measured 812.8 pg/mL.

PD-1/CD279 Uni-rAb^® Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83516-9-PBS

Clone No.240389D12

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CD279, PD-1, PD1, Pdc1, PD 1

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Product Information

83516-9-PBS targets PD-1/CD279 as part of a matched antibody pair:

MP00496-4: 83516-4-PBS capture and 83516-9-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: programmed cell death 1
• Calculated Molecular Weight: 32 kDa
• GenBank Accession Number: NM_008798.2
• Gene Symbol: PD-1
• Gene ID (NCBI): 18566
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q02242
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.