PD-ECGF Polyclonal antibody

PD-ECGF Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 12383-1-AP

Synonyms

ECGF1, Gliostatin, hPD ECGF, MNGIE, PD ECGF, PDECGF, PD-ECGF, TdRPase, thymidine phosphorylase, TP, TYMP



Tested Applications

Positive WB detected inA431 cells, HeLa cells, THP-1 cells
Positive IHC detected inhuman breast cancer tissue, human stomach cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12383-1-AP targets PD-ECGF in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PD-ECGF fusion protein Ag3052
Full Name thymidine phosphorylase
Calculated Molecular Weight 482 aa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC018160
Gene Symbol TYMP
Gene ID (NCBI) 1890
RRIDAB_2288047
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Platelet derived endothelial cell growth factor (PD-ECGF), also known as thymidine phosphorylase (TP), induces migration and angiogenesis in endothelial and tumor cells, and was upregulated in various malignancies compared to that in normal tissues. Interestingly, PD-ECGF has dual effect on tumor development and chemotherapy. It could stimulate cancer cell migration and proliferation. On the other hand, some chemotherapeutic agents (5-fluorouracil, capecitabine, etc.) were converted to their active forms through TP enzymes.

Protocols

Product Specific Protocols
WB protocol for PD-ECGF antibody 12383-1-APDownload protocol
IHC protocol for PD-ECGF antibody 12383-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncogene

The proteomic characterization of the peritumor microenvironment in human hepatocellular carcinoma.

Authors - Yuhan Gu
humanFC

Oncotarget

PMID: 29707135

Authors - Toshiki Iwai
mouseWB,IF

Acta Pharmacol Sin

Exploring the protective effects of schizandrol A in acute myocardial ischemia mice by comprehensive metabolomics profiling integrated with molecular mechanism studies.

Authors - Qiong Lai
mouseWB

J Ovarian Res

4-Methylumbelliferone inhibits ovarian cancer growth by suppressing thymidine phosphorylase expression.

Authors - Ryosuke Tamura
humanIHC

Mol Imaging Biol

Evaluation of [(18)F]Fluorothymidine as a Biomarker for Early Therapy Response in a Mouse Model of Colorectal Cancer.

Authors - Sara Rapic
mouseWB

Antioxidants (Basel)

D-Penicillamine Reveals the Amelioration of Seizure-Induced Neuronal Injury via Inhibiting Aqp11-Dependent Ferroptosis

Authors - Nan Yang