|Positive FC detected in||human peripheral blood lymphocytes|
|This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.|
|Sample-dependent, check data in validation data gallery|
APC-65081 targets PD-L1 (B7-H1) in FC applications and shows reactivity with Human samples.
|Host / Isotype||Mouse / IgG2b, kappa|
|Immunogen||Full length human PD-L1|
|Full Name||CD274 molecule|
|GenBank accession number||BC069381|
|Gene ID (NCBI)||29126|
|Conjugate||APC Fluorescent Dye|
|Excitation/Emission maxima wavelengths||650 nm / 660 nm|
|Purification Method||Affinity purification|
|Storage Buffer||Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.|
|Storage Conditions||Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.|
Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.
Adv Sci (Weinh)
Inhibition of CK2/ING4 Pathway Facilitates Non-Small Cell Lung Cancer Immunotherapy
Dual role of pseudogene TMEM198B in promoting lipid metabolism and immune escape of glioma cells
PPARγ inhibited tumor immune escape by inducing PD-L1 autophagic degradation
Stem Cell Res Ther
Glioma-associated mesenchymal stem cells-mediated PD-L1 expression is attenuated by Ad5-Ki67/IL-15 in GBM treatment.
Eur J Pharmacol
PPARγ agonist pioglitazone enhances colorectal cancer immunotherapy by inducing PD-L1 autophagic degradation