Mouse PD-L1/CD274 Recombinant antibody, PBS Only (Capture)

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  Sandwich ELISA standard curve of MP00844-2

  Sandwich ELISA standard curve of MP00844-2, Mouse PD-L1/CD274 Recombinant Matched Antibody Pair - PBS only. 83906-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0986. 83906-1-PBS was HRP conjugated as the detection antibody. Range: 4.69-300 pg/mL

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  Sample test of MP00844-2

  Serum of sixteen individual healthy human donors was measured. The mouse PD-L1/CD274 concentration of detected samples was determined to be 99.6 pg/mL with a range of 40.12 - 315.13 pg/mL.

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  Sample test of MP00844-2

  RAW264.7 and NIH/3T3 cell lysate were measured. The mouse PD-L1/CD274 concentration was determined to be 918.7 pg/mL in RAW264.7 cell lysate (based on a 4.8 mg/mL extract load) and 144.6 pg/mL in NIH/3T3 cell lysate (based on a 10.6 mg/mL extract load).

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  Cytometric bead array standard curve of MP00844-2

  Cytometric bead array standard curve of MP00844-2, MOUSE PD-L1/CD274 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83906-4-PBS. Detection antibody: 83906-1-PBS. Standard: Eg0986. Range: 0.313-40 ng/mL.

PD-L1/CD274 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83906-4-PBS

Clone No.240993H1

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Cd274, PD-L1, Pdcd1lg1, Pdcd1l1, PD L1

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Product Information

83906-4-PBS targets PD-L1/CD274 as part of a matched antibody pair:

MP00844-2: 83906-4-PBS capture and 83906-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD274 antigen
• Calculated Molecular Weight: 33 kDa
• GenBank Accession Number: NM_021893.3
• Gene Symbol: PD-L1
• Gene ID (NCBI): 60533
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9EP73
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.