Mouse PD-L1/CD274 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83906-6-RR (same clone as 83906-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83906-6-RR (PD-L1/CD274 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83906-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 83906-6-RR (same clone as 83906-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83906-6-RR (PD-L1/B7-H1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83906-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 83906-6-RR (same clone as 83906-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83906-6-RR (PD-L1/B7-H1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83906-6-PBS in a different storage buffer formulation.

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  IF Staining of human placenta using 83906-6-RR (same clone as 83906-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using PD-L1/CD274 antibody (83906-6-RR, Clone: 240993C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83906-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83906-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83906-6-RR against Mouse PD-L1/B7-H1 were performed. The affinity constant is below 1 pM.

PD-L1/CD274 Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 83906-6-PBS

Clone No.240993C9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Cd274, PD-L1, 240993C9, B7 H1, PD L1

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Product Information

83906-6-PBS targets PD-L1/CD274 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0986

  Product name: Recombinant Mouse PD-L1/B7-H1 protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 19-239 aa of NM_021893.3

  Sequence: FTITAPKDLYVVEYGSNVTMECRFPVERELDLLALVVYWEKEDEQVIQFVAGEEDLKPQHSNFRGRASLPKDQLLKGNAALQITDVKLQDAGVYCCIISYGGADYKRITLKVNAPYRKINQRISVDPATSEHELICQAEGYPEAEVIWTNSDHQPVSGKRSVTTSRTEGMLLNVTSSLRVNATANDVFYCTFWRSQPGQNHTAELIIPELPATHPPQNRTH

• Full Name: CD274 antigen
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: NM_021893.3
• Gene Symbol: PD-L1
• Gene ID (NCBI): 60533
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9EP73
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.