PD-L1/CD274 Recombinant monoclonal antibody

• 

  WB analysis of human placenta using 85229-23-RR

  human placenta tissue was subjected to SDS PAGE followed by western blot with 85229-23-RR (PD-L1/CD274 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-231 using 85229-23-RR

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 85229-23-RR (PD-L1/CD274 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-87 MG using 85229-23-RR

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 85229-23-RR (PD-L1/CD274 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 85229-23-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 85229-23-RR (PD-L1/CD274 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 85229-23-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 85229-23-RR (PD-L1/CD274 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 85229-23-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85229-23-RR (PD-L1/CD274 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 85229-23-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85229-23-RR (PD-L1/CD274 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PD-L1/CD274 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 85229-23-RR

Clone No.251887E5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CD274, PD-L1, B7 H1, B7-H1, hPD-L1

---

Tested Applications

• Positive WB detected in: human placenta tissue, MDA-MB-231 cells, U-87 MG cells
• Positive IHC detected in: human tonsillitis tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85229-23-RR targets PD-L1/CD274 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: CD274 molecule
• Calculated Molecular Weight: 290 aa, 33 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC074984
• Gene Symbol: PD-L1
• Gene ID (NCBI): 29126
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NZQ7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.