• Featured Product
  • KD/KO Validated

PDCD2 Polyclonal antibody

PDCD2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, zebrafish

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10725-1-AP

Synonyms

PDCD2, programmed cell death 2, RP8, Zinc finger protein Rp 8, ZMYND7



Tested Applications

Positive WB detected inHeLa cells, H7ES cells, mouse liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10725-1-AP targets PDCD2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, zebrafish samples.

Tested Reactivity human, mouse, zebrafish
Cited Reactivityhuman, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDCD2 fusion protein Ag1139
Full Name programmed cell death 2
Calculated molecular weight 39 kDa
Observed molecular weight 37-39 kDa, 45 kDa
GenBank accession numberBC008378
Gene symbol PDCD2
Gene ID (NCBI) 5134
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Programmed Cell Death 2 (PDCD2) is widely expressed in embryonic and adult tissues but with undefined function yet. Recent observation uncovered its role in hematopoietic stem cell differentiation during development. Catalog# 10725-1-AP is a rabbit polyclonal antibody raised against full-length PDCD2 of human origin.

Protocols

Product Specific Protocols
WB protocol for PDCD2 antibody 10725-1-APDownload protocol
IHC protocol for PDCD2 antibody 10725-1-APDownload protocol
IP protocol for PDCD2 antibody 10725-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IHC

Exp Hematol

PDCD2 knockdown inhibits erythroid but not megakaryocytic lineage differentiation of human hematopoietic stem/progenitor cells.

Authors - Kokorina Natalia A NA
mouseWB

Dev Biol

PDCD2 is essential for inner cell mass development and embryonic stem cell maintenance.

Authors - Mu Weipeng W
  • KO Validated
human, mouse, zebrafishIHC

Stem Cells Dev

PDCD2 controls hematopoietic stem cell differentiation during development.

Authors - Kramer Joseph J
mouseIHC

Sci Rep

Single-cell RNA-seq reveals distinct injury responses in different types of DRG sensory neurons.

Authors - Ganlu Hu

Br J Haematol

MicroRNA 200a inhibits erythroid differentiation by targeting PDCD4 and THRB.

Authors - Yanming Li
human

Pharmacol Res

Chip-DSF: A rapid screening strategy for drug protein targets.

Authors - Zhao Cui