PDCD2 Polyclonal antibody

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PDCD2 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 10725-1-AP Copied!

Publications (9)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, zebrafish and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

PDCD2, programmed cell death 2, RP8, Zinc finger protein Rp 8, ZMYND7

• 

  WB analysis of HeLa using 10725-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10725-1-AP (PDCD2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse liver using 10725-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10725-1-AP (PDCD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of H7ES using 10725-1-AP

  H7ES cells were subjected to SDS PAGE followed by western blot with 10725-1-AP (PDCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 10725-1-AP

  IP result of anti-PDCD2 (IP:10725-1-AP, 4ug; Detection:10725-1-AP 1:500) with HeLa cells lysate 2800ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary tumor using 10725-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10725-1-AP (PDCD2 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, H7ES cells, mouse liver tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10725-1-AP targets PDCD2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, zebrafish samples.

• Tested Reactivity: human, mouse, zebrafish
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1139

  Product name: Recombinant human PDCD2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-344 aa of BC008378

  Sequence: MAAAGARPVELGFAESAPAWRLRSEQFPSKVGGRPAWLGAAGLPGPQALACELCGRPLSFLLQVYAPLPGRPDAFHRCIFLFCCREQPCCAGLRVFRNQLPRKNDFYSYEPPSENPPPETGESVCLQLKSGAHLCRVCGCLGPKTCSRCHKAYYCSKEHQTLDWRLGHKQACAQPDHLDHIIPDHNFLFPEFEIVIETEDEIMPEVVEKEDYSEIIGSMGEALEEELDSMAKHESREDKIFQKFKTQIALEPEQILRYGRGIAPIWISGENIPQEKDIPDCPCGAKRILEFQVMPQLLNYLKADRLGKSIDWGILAVFTCAESCSLGTGYTEEFVWKQDVTDTP

• Full Name: programmed cell death 2
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 37-39 kDa, 45 kDa
• GenBank Accession Number: BC008378
• Gene Symbol: PDCD2
• Gene ID (NCBI): 5134
• RRID: AB_2236895
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16342
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Programmed Cell Death 2 (PDCD2) is widely expressed in embryonic and adult tissues but with undefined function yet. Recent observation uncovered its role in hematopoietic stem cell differentiation during development. Catalog# 10725-1-AP is a rabbit polyclonal antibody raised against full-length PDCD2 of human origin.

Publications

What published studies show

The anti-PDCD2 antibody (Cat# 10725-1-AP) has 9 citations across journals including Genes Dis, Pharmacol Res, Mol Cell Proteomics, Sci Rep, Br J Haematol, Exp Hematol, Dev Biol, Stem Cells Dev, and Int J Clin Exp Pathol. Research fields span glioma prognosis, drug target screening, proteomics, sensory neuron injury, erythroid differentiation, hematopoietic stem cell development, embryonic stem cell maintenance, and osteosarcoma tumor suppression.

Automatically generated from the 9 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Genes Dis; PDCD2 as a prognostic biomarker in glioma correlates with malignant phenotype; Authors - Fengsheng Dai
human		Pharmacol Res; Chip-DSF: A rapid screening strategy for drug protein targets.; Authors - Zhao Cui
human	WB	Mol Cell Proteomics; Quantitative proteomic analysis of cellular protein modulation upon inhibition of the NEDD8-activating enzyme by MLN4924.; Authors - Hua Liao
mouse	IHC	Sci Rep; Single-cell RNA-seq reveals distinct injury responses in different types of DRG sensory neurons.; Authors - Ganlu Hu
		Br J Haematol; MicroRNA 200a inhibits erythroid differentiation by targeting PDCD4 and THRB.; Authors - Yanming Li
human	WB, IHC	Exp Hematol; PDCD2 knockdown inhibits erythroid but not megakaryocytic lineage differentiation of human hematopoietic stem/progenitor cells.; Authors - Kokorina Natalia A NA