PDE8A Polyclonal antibody

• 

  WB analysis of mouse testis using 13956-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13956-1-AP (PDE8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 13956-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13956-1-AP (PDE8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 13956-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13956-1-AP (PDE8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 13956-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13956-1-AP (PDE8A antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 13956-1-AP

  IP result of anti-PDE8A (IP:13956-1-AP, 5ug; Detection:13956-1-AP 1:1000) with mouse testis tissue lysate 8000ug.

• 

  IHC staining of human colon cancer using 13956-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 13956-1-AP (PDE8A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon cancer using 13956-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 13956-1-AP (PDE8A antibody) at dilution of 1:100 (under 40x lens).

PDE8A Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 13956-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, ELISA

Synonyms

HsT19550, PDE8A, phosphodiesterase 8A

---

Tested Applications

• Positive WB detected in: mouse testis tissue, mouse spleen tissue, SH-SY5Y cells
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 3 publications below

Product Information

13956-1-AP targets PDE8A in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5069

  Product name: Recombinant human PDE8A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 530-829 aa of BC060762

  Sequence: FLHCSESTLRSWLQIIEANYHSSNPYHNSTHSADVLHATAYFLSKERIKETLDPIDEVAALIAATIHDVDHPGRTNSFLCNAGSELAILYNDTAVLESHHAALAFQLTTGDDKCNIFKNMERNDYRTLRQGIIDMVLATEMTKHFEHVNKFVNSINKPLATLEENGETDKNQEVINTMLRTPENRTLIKRMLIKCADVSNPCRPLQYCIEWAARISEEYFSQTDEEKQQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFVDLPDLMQHLDNNFKYWKGLDEMKLRNLRPPPE

• Full Name: phosphodiesterase 8A
• Calculated Molecular Weight: 93 kDa
• Observed Molecular Weight: 93 kDa, 88 kDa
• GenBank Accession Number: BC060762
• Gene Symbol: PDE8A
• Gene ID (NCBI): 5151
• RRID: AB_2161728
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60658
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDE8A(high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A) belongs to the cyclic nucleotide phosphodiesterase family and PDE8 subfamily. PDE8A plays a critical role in the modulation of at least one compartment of cAMP and hence PKA activity during β-adrenergic receptor (βAR) activation in ventricular myocytes(PMID:20353794). The full length protein contains several sites for protein phosphorylation, N-glycosylation, N-myristoylation, and amidation. It has 5 isoforms produced by alternative splicing with the molecular mass of 93 kDa, 88 kDa, 51 kDa and the isoform 4 and 5 have the same molecular mass of 66 kDa.(PMID:11738832).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Mol Metab; Inhibition of prolyl hydroxylases increases hepatic insulin and decreases glucagon sensitivity by an HIF-2α-dependent mechanism.; Authors - Matthew Riopel
pig	WB,IF	Sci Rep; Mitochondrial sub-cellular localization of cAMP-specific phosphodiesterase 8A in ovarian follicular cells.; Authors - Amel Lounas
		Reprod Fertil Dev; Active 3'-5' cyclic nucleotide phosphodiesterases are present in detergent-resistant membranes of mural granulosa cells.; Authors - Annick Bergeron
human	IF	Sci Rep; Disruption of the pro-oncogenic c-RAF-PDE8A complex represents a differentiated approach to treating KRAS-c-RAF dependent PDAC; Authors - Sean F Cooke
mouse	WB,IF	Neurochem Res; Phosphodiesterase 8 (PDE8): Distribution and Cellular Expression and Association with Alzheimer's Disease; Authors - Nian-Zhuang Qiu