PDE9A Polyclonal antibody

PDE9A Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12648-1-AP

Synonyms

HSPDE9A2, PDE9A, phosphodiesterase 9A

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Validation Data Gallery

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  IHC staining of human prostate cancer using 12648-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12648-1-AP (PDE9A Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human prostate cancer using 12648-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12648-1-AP (PDE9A Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human prostate cancer using 12648-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12648-1-AP (PDE9A antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of A549 using 12648-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 12648-1-AP (PDE9A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A549 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12648-1-AP targets PDE9A in IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDE9A fusion protein Ag3372
• Full Name: phosphodiesterase 9A
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC009047
• Gene Symbol: PDE9A
• Gene ID (NCBI): 5152
• RRID: AB_2161890
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclic nucleotide phosphodiesterases (PDEs), which hydrolyze the intracellular second messengers cAMP and cGMP to their corresponding monophosphates, play an important role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. PDE9A plays a role in signal transduction by regulating the intracellular concentration of these cyclic nucleotides. Multiple transcript variants encoding several different isoforms have been found for this gene. This protein has 16 isoforms with the MW between 44 kDa and 65 kDa.

Publications

Species	Application	Title
human,rat		Eur J Pharmacol; A novel phosphodiesterase 9A inhibitor LW33 protects against ischemic stroke through the cGMP/PKG/CREB pathway.; Authors - Jia-Yi You