PDGF-D Recombinant monoclonal antibody

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• PDGF-D Recombinant monoclonal antibody (81718-2-RR)

• 

  WB analysis using 81718-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81718-2-RR (PDGFD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  Affinity of 81718-2-RR

  Biolayer interferometry (BLl) kinetic assays of 81718-2-RR against Human PDGF-D were performed. The affinity constant is 10.4 nM.

PDGF-D Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 81718-2-RR

Clone No.252468G6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

PDGFD, Iris-expressed growth factor, PDGF D, PDGFD receptor binding form, Platelet-derived growth factor D

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Tested Applications

• Positive WB detected in: mouse heart tissue, human heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

81718-2-RR targets PDGF-D in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg6204

  Product name: Recombinant human PDGFD protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 19-370 aa of NM_025208.4

  Sequence: RDTSATPQSASIKALRNANLRRDESNHLTDLYRRDETIQVKGNGYVQSPRFPNSYPRNLLLTWRLHSQENTRIQLVFDNQFGLEEAENDICRYDFVEVEDISETSTIIRGRWCGHKEVPPRIKSRTNQIKITFKSDDYFVAKPGFKIYYSLLEDFQPAAASETNWESVTSSISGVSYNSPSVTDPTLIADALDKKIAEFDTVEDLLKYFNPESWQEDLENMYLDTPRYRGRSYHDRKSKVDLDRLNDDAKRYSCTPRNYSVNIREELKLANVVFFPRCLLVQRCGGNCGCGTVNWRSCTCNSGKTVKKYHEVLQFEPGHIKRRGRAKTMALVDIQLDHHERCDCICSSRPPR

• Full Name: platelet derived growth factor D
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: NM_025208.4
• Gene Symbol: PDGFD
• Gene ID (NCBI): 80310
• RRID: AB_3743391
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9GZP0-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Platelet-derived growth factor-D (PDGF-D) is the newest member of the PDGF family. It is secreted as a latent dimer that must undergo extracellular proteolytic removal of its N-terminal CUB domain before the growth-factor domain can activate the β-PDGFR. Once activated, PDGF-D drives proliferation, migration and survival of mesenchymal cells and promotes angiogenesis, wound healing and tissue remodeling. Clinically, PDGF-D is up-regulated in several cancers and in fibrotic diseases of the heart, kidney and lung, making it a potential therapeutic target.