PDGFR beta/CD140b Monoclonal antibody, PBS Only

• Home
• Products
• Primary Antibodies
• PDGFR beta/CD140b Monoclonal antibody, PBS Only (60535-13-PBS)

PDGFR beta/CD140b Monoclonal Antibody for WB, Indirect ELISA

Cat No. 60535-13-PBS

Clone No.4E2D5

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

PDGFRB, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, CD140B

• 

  WB analysis using 60535-13-Ig (same clone as 60535-13-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60535-13-Ig (PDGFR beta/CD140b antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60535-13-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of U2OS using 60535-13-Ig (same clone as 60535-13-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 60535-13-Ig (PDGFR beta/CD140b antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60535-13-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

---

Product Information

60535-13-PBS targets PDGFR beta/CD140b in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Eg0147

  Product name: Recombinant Human PDGFR beta/CD140b protein (Myc Tag, His Tag)

  Source: mammalian cells-derived, pHZ-KIsec

  Tag: Myc & 6*His

  Domain: 33-531 aa of BC032224

  Sequence: LVVTPPGPELVLNVSSTFVLTCSGSAPVVWERMSQEPPQEMAKAQDGTFSSVLTLTNLTGLDTGEYFCTHNDSRGLETDERKRLYIFVPDPTVGFLPNDAEELFIFLTEITEITIPCRVTDPQLVVTLHEKKGDVALPVPYDHQRGFFGIFEDRSYICKTTIGDREVDSDAYYVYRLQVSSINVSVNAVQTVVRQGENITLMCIVIGNEVVNFEWTYPRKESGRLVEPVTDFLLDMPYHIRSILHIPSAELEDSGTYTCNVTESVNDHQDEKAINITVVESGYVRLLGEVGTLQFAELHRSRTLQVVFEAYPPPTVLWFKDNRTLGDSSAGEIALSTRNVSETRYVSELTLVRVKVAEAGHYTMRAFHEDAEVQLSFQLQINVPVRVLELSESHPDSGEQTVRCRGRGMPQPNIIWSACRDLKRCPRELPPTLLGNSSEEESQLETNVTYWEEEQEFEVVSTLRLQHVDRPLSVRCTLRNAVGQDTQEVIVVPHSLPFK

• Full Name: platelet-derived growth factor receptor, beta polypeptide
• Calculated Molecular Weight: 1106 aa, 124 kDa
• Observed Molecular Weight: 160-180 kDa
• GenBank Accession Number: BC032224
• Gene Symbol: PDGFR beta
• Gene ID (NCBI): 5159
• RRID: AB_3743219
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P09619
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PDGFRs are receptor tyrosine kinases with an extracellular ligand-binding domain and a cytoplasmic enzymatic domain. The signaling activity of PDGFRs is tightly regulated by an autoinhibitory allosteric conformation, and requires ligand-induced receptor dimerization at the cell surface to alleviate autoinhibition and achieve physiological activity (PMID: 21664579). PDGFR beta (platelet-derived growth factor receptor- β)/CD140b is a constitutive marker expressed by ASCs in the angiogenic behavior of human retinal endothelial cells (HREs). CD140b (PDGFRβ) plays a crucial role in controlling the intrinsic proliferation (PMID: 30976657)