Tested Applications
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10945-1-AP targets PDGFR alpha in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1385 Product name: Recombinant human PDGFRA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-209 aa of BC015186 Sequence: MGTSHPAFLVLGCLLTGLSLILCQLSLPSILPNENEKVVQLNSSFSLRCFGESEVSWQYPMSEEESSDVEIRNEENNSGLFVTVLEVSSASAAHTGLYTCYYNHTQTEENELEGRHIYIYVPDPDVAFVPLGMTDYLVIVEDDDSAIIPCRTTDPETPVTLHNSEGVVPASYDSRQGFNGTFTVGPYICEATVKGKKFQTIPFNVYALK Predict reactive species |
| Full Name | platelet-derived growth factor receptor, alpha polypeptide |
| Calculated Molecular Weight | 123 kDa |
| GenBank Accession Number | BC015186 |
| Gene Symbol | PDGFR alpha |
| Gene ID (NCBI) | 5156 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P16234 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Platelet-derived growth factor receptor alpha (PDGFRA, also known as CD140a) is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. PDGFRA is implicated in the regulation of embryonic development, tumorigenesis, cell proliferation, survival, and chemotaxis. Mutations in the PDGFRA gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.
Publications
What published studies show
The PDGFR alpha Polyclonal antibody (Cat# 10945-1-AP) has 1 citation. It appears in Oncology Research (2023), a study investigating how GIPC1 promotes tumor growth and migration in gastric cancer through activation of the PDGFR/PI3K/AKT signaling pathway. The research field is oncology, specifically gastric cancer and growth factor receptor signaling.
| Species | Application | Title |
|---|---|---|
Oncol Res GIPC1 promotes tumor growth and migration in gastric cancer via activating PDGFR/PI3K/AKT signaling |
